• Immunoprecipitation analysis of 600 Mu g extracts of 293T cells using 3 Mu g [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568). Western blot was performed from the immunoprecipitate using CDX2 antibody (STJ11101568) at a dilution of 1:500.
  • Confocal imaging of Human colon using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.
  • Immunohistochemistry analysis of CDX2 in paraffin-embedded human colon carcinoma using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CDX2 in paraffin-embedded human appendix using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and CDX2 knockdown (KD) 293T cells, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CDX2 antibody (1-100) [S8MR] (STJ11101568)

SKU:
STJ11101568

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CDX2 (1-100) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDX2
Gene ID: 1045
Uniprot ID: CDX2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDX2 (Q99626).
Immunogen Sequence: MYVSYLLDKDVSMYPSSVRH SGGLNLAPQNFVSPPQYPDY GGYHVAAAAAAAANLDSAQS PGPSWPAAYGAPLREDWNGY APGGAAAAANAVAHGLNGGS
Tissue Specificity Detected in small intestine, colon and pancreas.
Post Translational Modifications Ubiquitinated, leading to its degradation by the proteasome. Phosphorylation at Ser-60 reduces transactivation capacity. Phosphorylation at Ser-283 reduces transactivation capacity and also increases ubiquitin-dependent proteasome degradation.
Function Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium. Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription. Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription. Binds to and activates transcription of LPH. Activates transcription of CLDN2 and intestinal mucin MUC2. Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA.
Protein Name Homeobox Protein Cdx-2
Cdx-3
Caudal-Type Homeobox Protein 2
Database Links Reactome: R-HSA-381771
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Cdx-2 antibody
Anti-Cdx-3 antibody
Anti-Caudal-Type Homeobox Protein 2 antibody
Anti-CDX2 antibody
Anti-CDX3 antibody

Information sourced from Uniprot.org

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