| Tissue Specificity | Detected in small intestine, colon and pancreas. |
| Post Translational Modifications | Ubiquitinated, leading to its degradation by the proteasome. Phosphorylation at Ser-60 reduces transactivation capacity. Phosphorylation at Ser-283 reduces transactivation capacity and also increases ubiquitin-dependent proteasome degradation. |
| Function | Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium. Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription. Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription. Binds to and activates transcription of LPH. Activates transcription of CLDN2 and intestinal mucin MUC2. Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA. |
| Protein Name | Homeobox Protein Cdx-2Cdx-3Caudal-Type Homeobox Protein 2 |
| Database Links | Reactome: R-HSA-381771 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Cdx-2 antibodyAnti-Cdx-3 antibodyAnti-Caudal-Type Homeobox Protein 2 antibodyAnti-CDX2 antibodyAnti-CDX3 antibody |
Information sourced from Uniprot.org
![Immunoprecipitation analysis of 600 Mu g extracts of 293T cells using 3 Mu g [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568). Western blot was performed from the immunoprecipitate using CDX2 antibody (STJ11101568) at a dilution of 1:500.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90481/359933/STJ11101568_1__84936.1713124490.jpg?c=1 "Immunoprecipitation analysis of 600 Mu g extracts of 293T cells using 3 Mu g [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568). Western blot was performed from the immunoprecipitate using CDX2 antibody (STJ11101568) at a dilution of 1:500.")
![Confocal imaging of Human colon using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90481/359934/STJ11101568_2__31874.1713124491.jpg?c=1 "Confocal imaging of Human colon using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.")
![Immunohistochemistry analysis of CDX2 in paraffin-embedded human colon carcinoma using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90481/359935/STJ11101568_3__94929.1713124491.jpg?c=1 "Immunohistochemistry analysis of CDX2 in paraffin-embedded human colon carcinoma using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of CDX2 in paraffin-embedded human appendix using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90481/359936/STJ11101568_4__07299.1713124492.jpg?c=1 "Immunohistochemistry analysis of CDX2 in paraffin-embedded human appendix using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and CDX2 knockdown (KD) 293T cells, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90481/359937/STJ11101568_5__49487.1713124493.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CDX2 knockdown (KD) 293T cells, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of various lysates using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90481/359938/STJ11101568_6__26475.1713124493.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunoprecipitation analysis of 600 Mu g extracts of 293T cells using 3 Mu g [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568). Western blot was performed from the immunoprecipitate using CDX2 antibody (STJ11101568) at a dilution of 1:500.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/90481/359933/STJ11101568_1__84936.1713124490.jpg?c=1 "Immunoprecipitation analysis of 600 Mu g extracts of 293T cells using 3 Mu g [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11101568). Western blot was performed from the immunoprecipitate using CDX2 antibody (STJ11101568) at a dilution of 1:500.")
