Western blot analysis of various lysates using Cdx1 Rabbit mAb (STJ11103662) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Cdx1 antibody (1-100) [S2MR] (STJ11103662)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103662
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General Information

Short DescriptionRabbit monoclonal Cdx1 (1-100) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS2MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCDX1
Gene ID1044
Uniprot IDCDX1_HUMAN
Immunogen Region1-100
Immunogen SequenceMYVGYVLDKDSPVYPGPARP ASLGLGPQAYGPPAPPPAPP QYPDFSSYSHVEPAPAPPTA WGAPFPAPKDDWAAAYGPGP AAPAASPASLAFGPPPDFSP
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cdx1 (P47902).

Additional Info

Tissue Specificity Intestinal epithelium.
Function Plays a role in transcriptional regulation. Involved in activated KRAS-mediated transcriptional activation of PRKD1 in colorectal cancer (CRC) cells. Binds to the PRKD1 promoter in colorectal cancer (CRC) cells. Could play a role in the terminal differentiation of the intestine. Binds preferentially to methylated DNA.
Protein Name Homeobox Protein Cdx-1
Caudal-Type Homeobox Protein 1
Database Links
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Cdx-1 antibody
Anti-Caudal-Type Homeobox Protein 1 antibody
Anti-CDX1 antibody

Information sourced from Uniprot.org

Citations

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