• Immunofluorescence analysis of NIH-3T3 cells using CDKN2D Rabbit polyclonal antibody (STJ28639) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CDKN2D Rabbit polyclonal antibody (STJ28639) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 90s.

Anti-CDKN2D antibody (1-166) (STJ28639)

SKU:
STJ28639

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDKN2D (1-166) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDKN2D
Gene ID: 1032
Uniprot ID: CDN2D_HUMAN
Immunogen Region: 1-166
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human CDKN2D (NP_001791.1).
Immunogen Sequence: MLLEEVRAGDRLSGAAARGD VQEVRRLLHRELVHPDALNR FGKTALQVMMFGSTAIALEL LKQGASPNVQDTSGTSPVHD AARTGFLDTLKVLVEHGADV NVPDGTGALPIHLAVQEGHT AVVSFLAAESDLHRRDARGL TPLELALQRGAQDLVDILQG HMVAPL
Function Interacts strongly with CDK4 and CDK6 and inhibits them.
Protein Name Cyclin-Dependent Kinase 4 Inhibitor D
P19-Ink4d
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559585
Reactome: R-HSA-69231
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 4 Inhibitor D antibody
Anti-P19-Ink4d antibody
Anti-CDKN2D antibody

Information sourced from Uniprot.org

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