![Confocal imaging of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit monoclonal antibody (STJ113253, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/92819/373410/STJ113253_1__34054.1713139652.jpg?c=1 "Confocal imaging of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit monoclonal antibody (STJ113253, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Immunohistochemistry analysis of CDKN2A/p16INK4a in paraffin-embedded human cervix cancer using [KO Validated] CDKN2A/p16INK4a Rabbit monoclonal antibody (STJ113253) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/92819/373411/STJ113253_2__22383.1713139653.jpg?c=1 "Immunohistochemistry analysis of CDKN2A/p16INK4a in paraffin-embedded human cervix cancer using [KO Validated] CDKN2A/p16INK4a Rabbit monoclonal antibody (STJ113253) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Confocal imaging of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit monoclonal antibody (STJ113253, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/92819/373410/STJ113253_1__34054.1713139652.jpg?c=1 "Confocal imaging of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit monoclonal antibody (STJ113253, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
| Short Description : | Rabbit monoclonal CDKN2A/p16INK4a (57-156) antibody for use in WB, IHC-P, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S3MR |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:2000IHC-P:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDKN2A |
| Gene ID: | 1029 |
| Uniprot ID: | CDN2A_HUMAN |
| Immunogen Region: | 57-156 |
| Immunogen Sequence: | ARVAELLLLHGAEPNCADPA TLTRPVHDAAREGFLDTLVV LHRAGARLDVRDAWGRLPVD LAEELGHRDVARYLRAAAGG TRGSNHARIDAAEGPSDIPD |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 57-156 of human CDKN2A/p16INK4a (P42771). |
| Tissue Specificity | Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific. |
| Post Translational Modifications | Phosphorylation seems to increase interaction with CDK4. |
| Function | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. |
| Protein Name | Cyclin-Dependent Kinase Inhibitor 2aCyclin-Dependent Kinase 4 Inhibitor ACdk4iMultiple Tumor Suppressor 1Mts-1P16-Ink4aP16-Ink4P16ink4a |
| Database Links | Reactome: R-HSA-111471Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-3108214Reactome: R-HSA-3232118Reactome: R-HSA-6804757Reactome: R-HSA-69541Reactome: R-HSA-8941858Reactome: R-HSA-9645722Reactome: R-HSA-9646303Reactome: R-HSA-9646304Reactome: R-HSA-9759194Reactome: R-HSA-2559582Reactome: R-HSA-69231Reactome: R-HSA-8853884Reactome: R-HSA-9630791Reactome: R-HSA-9630794Reactome: R-HSA-9632697Reactome: R-HSA-9632700Reactome: R-HSA-9825892 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase Inhibitor 2a antibodyAnti-Cyclin-Dependent Kinase 4 Inhibitor A antibodyAnti-Cdk4i antibodyAnti-Multiple Tumor Suppressor 1 antibodyAnti-Mts-1 antibodyAnti-P16-Ink4a antibodyAnti-P16-Ink4 antibodyAnti-P16ink4a antibodyAnti-CDKN2A antibodyAnti-CDKN2 antibodyAnti-MTS1 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ23076) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/99355/384261/STJ23076_1__86905.1713152939.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ23076) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
