Anti-CDKN1A antibody (Full Length) [M513] (STJA0005501)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenClone M513 was generated from a full length recombinant human p21 protein. The antibody detects human p21.
General Information
| Short Description | Mouse monoclonal antibody anti-p21/CIP1/WAF1 (Full Length) is suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation research applications. |
| Applications | WB/ICC/IHC/IP |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M513 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:500IHC 1:50ICC 1:50IP 1:50 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDKN1A |
| Gene ID | 1026 |
| Uniprot ID | CDN1A_HUMAN |
| Immunogen | Clone M513 was generated from a full length recombinant human p21 protein. The antibody detects human p21. |
| Immunogen Region | Full Length |
| Specificity | The antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of p21 on SDS-PAGE immunoblots of human endothelial cells (HUVEC) , HeLa cells, and Fibroblasts. The antibody is also useful for immunoprecipitation and immunofluores |
Additional Info
| Post Translational Modifications | Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. Ubiquitinated by MKRN1.leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114.leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1. |
| Function | Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes. |
| Protein Name | Cyclin-Dependent Kinase Inhibitor 1Cdk-Interacting Protein 1Melanoma Differentiation-Associated Protein 6Mda-6P21 |
| Database Links | Reactome: R-HSA-187577Reactome: R-HSA-198323Reactome: R-HSA-2559582Reactome: R-HSA-2559586Reactome: R-HSA-5674400Reactome: R-HSA-6785807Reactome: R-HSA-6804116Reactome: R-HSA-69202Reactome: R-HSA-69231Reactome: R-HSA-69563Reactome: R-HSA-69656Reactome: R-HSA-69895Reactome: R-HSA-8852276Reactome: R-HSA-8866911Reactome: R-HSA-8878166Reactome: R-HSA-8941855Reactome: R-HSA-8951664Reactome: R-HSA-9616222Reactome: R-HSA-9617828Reactome: R-HSA-9661069Reactome: R-HSA-9702518Reactome: R-HSA-9703465Reactome: R-HSA-9725370Reactome: R-HSA-9755511Reactome: R-HSA-9825892 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase Inhibitor 1 antibodyAnti-Cdk-Interacting Protein 1 antibodyAnti-Melanoma Differentiation-Associated Protein 6 antibodyAnti-Mda-6 antibodyAnti-P21 antibodyAnti-CDKN1A antibodyAnti-CAP20 antibodyAnti-CDKN1 antibodyAnti-CIP1 antibodyAnti-MDA6 antibodyAnti-PIC1 antibodyAnti-SDI1 antibodyAnti-WAF1 antibody |
Information sourced from Uniprot.org