• Western blot analysis of extracts of various cell lines, using CDKN1A antibody (STJ11103324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CDKN1A antibody (1-159) (STJ11103324)

SKU:
STJ11103324

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDKN1A (1-159) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Cdkn1a
Gene ID: 12575
Uniprot ID: CDN1A_MOUSE
Immunogen Region: 1-159
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-159 of mouse CDKN1A (NP_031695.1).
Immunogen Sequence: MSNPGDVRPVPHRSKVCRCL FGPVDSEQLRRDCDALMAGC LQEARERWNFDFVTETPLEG NFVWERVRSLGLPKVYLSPG SRSRDDLGGDKRPSTSSALL QGPAPEDHVALSLSCTLVSE RPEDSPGGPGTSQGRKRRQT SLTDFYHSKRRLVFCKRKP
Post Translational Modifications Phosphorylation of Thr-140 or Ser-141 impairs binding to PCNA. Phosphorylation at Ser-112 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-140 by PIM2 enhances its stability and inhibits cell proliferation. Phosphorylation of Thr-140 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Ser-78 and Ser-141 by NUAK1 leads to its degradation. Ubiquitinated by MKRN1.leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114.leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-136, Lys-149, Lys-156 and Lys-158. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1.
Function May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.
Protein Name Cyclin-Dependent Kinase Inhibitor 1
Cdk-Interacting Protein 1
Melanoma Differentiation-Associated Protein
P21
Database Links Reactome: R-MMU-187577
Reactome: -MMU-198323
Reactome: -MMU-2559582
Reactome: -MMU-2559586
Reactome: -MMU-6804116
Reactome: -MMU-69202
Reactome: -MMU-69231
Reactome: -MMU-69563
Reactome: -MMU-69656
Reactome: -MMU-8852276
Reactome: -MMU-9616222
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Cyclin-Dependent Kinase Inhibitor 1 antibody
Anti-Cdk-Interacting Protein 1 antibody
Anti-Melanoma Differentiation-Associated Protein antibody
Anti-P21 antibody
Anti-Cdkn1a antibody
Anti-Cip1 antibody
Anti-Waf1 antibody

Information sourced from Uniprot.org

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