• STJ73141 (0. 3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CDK7 antibody (47-58) (STJ73141)

SKU:
STJ73141-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CDK7 (47-58) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CDK7
Gene ID: 1022
Uniprot ID: CDK7_HUMAN
Immunogen Region: 47-58
Accession Number: NP_001790.1
Immunogen Sequence: HRSEAKDGINRT
Post Translational Modifications Phosphorylation of Ser-164 during mitosis inactivates the enzyme. Phosphorylation of Thr-170 is required for activity. Phosphorylated at Ser-164 and Thr-170 by CDK2.
Function Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53.this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.
Protein Name Cyclin-Dependent Kinase 7
39 Kda Protein Kinase
P39 Mo15
Cdk-Activating Kinase 1
Cell Division Protein Kinase 7
Serine/Threonine-Protein Kinase 1
Tfiih Basal Transcription Factor Complex Kinase Subunit
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-167200
Reactome: R-HSA-167246
Reactome: R-HSA-427413
Reactome: R-HSA-5696395
Reactome: R-HSA-674695
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6796648
Reactome: R-HSA-6807505
Reactome: R-HSA-69202
Reactome: R-HSA-69231
Reactome: R-HSA-69273
Reactome: R-HSA-69656
Reactome: R-HSA-72086
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-73863
Reactome: R-HSA-75953
Reactome: R-HSA-75955
Reactome: R-HSA-76042
Reactome: R-HSA-77075
Reactome: R-HSA-8939236
Cellular Localisation Nucleus
Cytoplasm
Perinuclear Region
Colocalizes With Prkci In The Cytoplasm And Nucleus
Translocates From The Nucleus To Cytoplasm And Perinuclear Region In Response To Dna-Bound Peptides
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 7 antibody
Anti-39 Kda Protein Kinase antibody
Anti-P39 Mo15 antibody
Anti-Cdk-Activating Kinase 1 antibody
Anti-Cell Division Protein Kinase 7 antibody
Anti-Serine/Threonine-Protein Kinase 1 antibody
Anti-Tfiih Basal Transcription Factor Complex Kinase Subunit antibody
Anti-CDK7 antibody
Anti-CAK antibody
Anti-CAK1 antibody
Anti-CDKN7 antibody
Anti-MO15 antibody
Anti-STK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance