| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CDK5R1 (99-307) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CDK5R1 |
| Gene ID: |
8851 |
| Uniprot ID: |
CD5R1_HUMAN |
| Immunogen Region: |
99-307 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 99-307 of human CDK5R1 (NP_003876.1). |
| Immunogen Sequence: |
AQPPPAQPPAPPASQLSGSQ TGGSSSVKKAPHPAVTSAGT PKRVIVQASTSELLRCLGEF LCRRCYRLKHLSPTDPVLWL RSVDRSLLLQGWQDQGFITP ANVVFLYMLCRDVISSEVGS DHELQAVLLTCLYLSYSYMG NEISYPLKPFLVESCKEAFW DRCLSVINLMSSKMLQINAD PHYFTQVFSDLKNESGQEDK KRLLLGLDR |
| Tissue Specificity | Brain and neuron specific. |
| Post Translational Modifications | The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo. Myristoylated. A proper myristoylation signal is essential for the proper distribution of p35. Ubiquitinated, leading to its degradation: degradation of p35 by proteasome results in down-regulation of CDK5 activity. During this process, CDK5 phosphorylates p35 and induces its ubiquitination and subsequent degradation. Ubiquitinated by the CRL2(FEM1B) complex, which recognizes the -Gly-Leu-Asp-Arg C-degron at the C-terminus, leading to its degradation. Phosphorylation at Ser-8 and Thr-138 by CDK5 prevents calpain-mediated proteolysis. |
| Function | p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-BMAL1 heterodimer in association with altered stability and subcellular distribution. |
| Protein Name | Cyclin-Dependent Kinase 5 Activator 1Cdk5 Activator 1Cyclin-Dependent Kinase 5 Regulatory Subunit 1Tpkii Regulatory Subunit Cleaved Into - Cyclin-Dependent Kinase 5 Activator 1 - P35P35 - Cyclin-Dependent Kinase 5 Activator 1 - P25P25Tau Protein Kinase Ii 23 Kda SubunitP23 |
| Database Links | Reactome: R-HSA-399956Reactome: R-HSA-6804756Reactome: R-HSA-8862803Reactome: R-HSA-9031628Reactome: R-HSA-9032845Reactome: R-HSA-9768919 |
| Cellular Localisation | Cyclin-Dependent Kinase 5 Activator 1P35: Cell MembraneLipid-AnchorCytoplasmic SideCell ProjectionNeuron ProjectionIn The Primary Cortical NeuronsP35 Is Present In The Peripheries And Nerve TerminalsP25: NucleusCytoplasmPerinuclear RegionPerikaryonThe Conversion Of P35 To P25 Relocalizes The Protein From The Cell Periphery To The CytoplasmIn Nuclear And Perinuclear RegionsP25 Is Primarily Concentrated In The Cell Soma And Is Largely Absent From Neurites |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 5 Activator 1 antibodyAnti-Cdk5 Activator 1 antibodyAnti-Cyclin-Dependent Kinase 5 Regulatory Subunit 1 antibodyAnti-Tpkii Regulatory Subunit Cleaved Into - Cyclin-Dependent Kinase 5 Activator 1 - P35 antibodyAnti-P35 - Cyclin-Dependent Kinase 5 Activator 1 - P25 antibodyAnti-P25 antibodyAnti-Tau Protein Kinase Ii 23 Kda Subunit antibodyAnti-P23 antibodyAnti-CDK5R1 antibodyAnti-CDK5R antibodyAnti-NCK5A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
