• Western blot analysis of extracts of various cell lines, using CDK16 antibody (STJ117850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of U2OS cells using CDK16 antibody (STJ117850) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using CDK16 antibody (STJ117850) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CDK16 antibody (277-496) (STJ117850)

SKU:
STJ117850

£169.50 - £449.50
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Host: Rabbit
Applications: IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDK16 (277-496) is suitable for use in Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDK16
Gene ID: 5127
Uniprot ID: CDK16_HUMAN
Immunogen Region: 277-496
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 277-496 of human CDK16 (NP_006192.1).
Immunogen Sequence: CHRQKVLHRDLKPQNLLINE RGELKLADFGLARAKSIPTK TYSNEVVTLWYRPPDILLGS TDYSTQIDMWGVGCIFYEMA TGRPLFPGSTVEEQLHFIFR ILGTPTEETWPGILSNEEFK TYNYPKYRAEALLSHAPRLD SDGADLLTKLLQFEGRNRIS AEDAMKHPFFLSLGERIHKL PDTTSIFALKEIQLQKEASL RSSSMPDSGRPAFRVVDTEF
Tissue Specificity Detected in pancreas islets (at protein level). Detected in brain and pancreas.
Post Translational Modifications Phosphorylation at Ser-153 inhibits kinase activity.
Function Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development. Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
Protein Name Cyclin-Dependent Kinase 16
Cell Division Protein Kinase 16
Pctaire-Motif Protein Kinase 1
Serine/Threonine-Protein Kinase Pctaire-1
Cellular Localisation Cytoplasm
Cytoplasmic Vesicle
Secretory Vesicle
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Synapse
Synaptosome
Colocalizes With Insulin In Pancreas Islets
Recruited To The Cell Membrane By Ccny
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 16 antibody
Anti-Cell Division Protein Kinase 16 antibody
Anti-Pctaire-Motif Protein Kinase 1 antibody
Anti-Serine/Threonine-Protein Kinase Pctaire-1 antibody
Anti-CDK16 antibody
Anti-PCTAIRE1 antibody
Anti-PCTK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance