| Host: | Rabbit |
| Applications: | IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S4853RM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | IHC-P:1:100-1:500 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDK12 |
| Gene ID: | 51755 |
| Uniprot ID: | CDK12_HUMAN |
| Immunogen Region: | 420-516 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 420-516 of human CDK12 (NP_057591.2). |
| Immunogen Sequence: | SKGSPVFLPRKENSSVEAKD SGLESKKLPRSVKLEKSAPD TELVNVTHLNTEVKNSSDTG KVKLDENSEKHLVKDLKAQG TRDSKPIALKEEIVTPK |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylation at Thr-893 increases kinase activity. |
| Function | Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors. |
| Protein Name | Cyclin-Dependent Kinase 12 Cdc2-Related Kinase - Arginine/Serine-Rich Crkrs Cell Division Cycle 2-Related Protein Kinase 7 Cdc2-Related Protein Kinase 7 Cell Division Protein Kinase 12 Hcdk12 |
| Database Links | Reactome: R-HSA-6796648 |
| Cellular Localisation | Nucleus Nucleus Speckle Colocalized With Nuclear Speckles Throughout Interphase |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 12 antibody Anti-Cdc2-Related Kinase - Arginine/Serine-Rich antibody Anti-Crkrs antibody Anti-Cell Division Cycle 2-Related Protein Kinase 7 antibody Anti-Cdc2-Related Protein Kinase 7 antibody Anti-Cell Division Protein Kinase 12 antibody Anti-Hcdk12 antibody Anti-CDK12 antibody Anti-CRK7 antibody Anti-CRKRS antibody Anti-KIAA0904 antibody |
Information sourced from Uniprot.org