• Immunohistochemistry analysis of paraffin-embedded rat testis using CDK12 Rabbit monoclonal antibody (STJ11104853) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using CDK12 Rabbit monoclonal antibody (STJ11104853) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human spleen using CDK12 Rabbit monoclonal antibody (STJ11104853) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human heart using CDK12 Rabbit monoclonal antibody (STJ11104853) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK12 antibody (420-516) [S4853RM] (STJ11104853)

SKU:
STJ11104853

€183.13 - €799.38
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Host: Rabbit
Applications: IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cyclin-Dependent Kinase 12 (420-516) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4853RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDK12
Gene ID: 51755
Uniprot ID: CDK12_HUMAN
Immunogen Region: 420-516
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 420-516 of human CDK12 (NP_057591.2).
Immunogen Sequence: SKGSPVFLPRKENSSVEAKD SGLESKKLPRSVKLEKSAPD TELVNVTHLNTEVKNSSDTG KVKLDENSEKHLVKDLKAQG TRDSKPIALKEEIVTPK
Tissue Specificity Widely expressed.
Post Translational Modifications Phosphorylation at Thr-893 increases kinase activity.
Function Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
Protein Name Cyclin-Dependent Kinase 12
Cdc2-Related Kinase - Arginine/Serine-Rich
Crkrs
Cell Division Cycle 2-Related Protein Kinase 7
Cdc2-Related Protein Kinase 7
Cell Division Protein Kinase 12
Hcdk12
Database Links Reactome: R-HSA-6796648
Cellular Localisation Nucleus
Nucleus Speckle
Colocalized With Nuclear Speckles Throughout Interphase
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 12 antibody
Anti-Cdc2-Related Kinase - Arginine/Serine-Rich antibody
Anti-Crkrs antibody
Anti-Cell Division Cycle 2-Related Protein Kinase 7 antibody
Anti-Cdc2-Related Protein Kinase 7 antibody
Anti-Cell Division Protein Kinase 12 antibody
Anti-Hcdk12 antibody
Anti-CDK12 antibody
Anti-CRK7 antibody
Anti-CRKRS antibody
Anti-KIAA0904 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance