• Western blot analysis of extracts of Mouse brain, using VE Cadherin antibody (STJ23047) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of extracts of HUVEC cells, using VE Cadherin antibody (STJ23047) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CDH5 antibody (259-358) (STJ23047)

SKU:
STJ23047

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VE Cadherin (259-358) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH5
Gene ID: 1003
Uniprot ID: CADH5_HUMAN
Immunogen Region: 259-358
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 259-358 of human VE Cadherin (NP_001786.2).
Immunogen Sequence: TQTKYTFVVPEDTRVGTSVG SLFVEDPDEPQNRMTKYSIL RGDYQDAFTIETNPAHNEGI IKPMKPLDYEYIQQYSFIVE ATDPTIDLRYMSPPAGNRAQ
Tissue Specificity Endothelial tissues and brain.
Post Translational Modifications Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. O-glycosylated.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.
Protein Name Cadherin-5
7b4 Antigen
Vascular Endothelial Cadherin
Ve-Cadherin
Cd Antigen Cd144
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-5218920
Cellular Localisation Cell Junction
Cell Membrane
Single-Pass Type I Membrane Protein
Found At Cell-Cell Boundaries And Probably At Cell-Matrix Boundaries
Krit1 And Cdh5 Reciprocally Regulate Their Localization To Endothelial Cell-Cell Junctions
Alternative Antibody Names Anti-Cadherin-5 antibody
Anti-7b4 Antigen antibody
Anti-Vascular Endothelial Cadherin antibody
Anti-Ve-Cadherin antibody
Anti-Cd Antigen Cd144 antibody
Anti-CDH5 antibody

Information sourced from Uniprot.org

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