![Immunohistochemistry analysis of N-Cadherin in paraffin-embedded human liver tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90534/360131/STJ11101620_IHC_3__48546.1713124747.jpg?c=1 "Immunohistochemistry analysis of N-Cadherin in paraffin-embedded human liver tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of N-Cadherin in paraffin-embedded mouse heart tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90534/360132/STJ11101620_IHC_4__38085.1713124748.jpg?c=1 "Immunohistochemistry analysis of N-Cadherin in paraffin-embedded mouse heart tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of N-Cadherin in paraffin-embedded rat heart tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90534/360133/STJ11101620_IHC_5__68850.1713124749.jpg?c=1 "Immunohistochemistry analysis of N-Cadherin in paraffin-embedded rat heart tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of N-Cadherin in paraffin-embedded rat liver tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90534/360134/STJ11101620_IHC_6__20913.1713124750.jpg?c=1 "Immunohistochemistry analysis of N-Cadherin in paraffin-embedded rat liver tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Confocal imaging of paraffin-embedded rat heart using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90534/360135/STJ11101620_CFM_7__98245.1713124750.jpg?c=1 "Confocal imaging of paraffin-embedded rat heart using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.")
![Confocal imaging of paraffin-embedded mouse heart using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90534/360136/STJ11101620_CFM_8__53037.1713124751.jpg?c=1 "Confocal imaging of paraffin-embedded mouse heart using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.")
Anti-CDH2 antibody (100-200) [S0MR] (STJ11101620)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal N-Cadherin (100-200) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S0MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:200-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDH2 |
| Gene ID | 1000 |
| Uniprot ID | CADH2_HUMAN |
| Immunogen Region | 100-200 |
| Immunogen Sequence | AKFLIYAQDKETQEKWQVAV KLSLKPTLTEESVKESAEVE EIVFPRQFSKHSGHLQRQKR DWVIPPINLPENSRGPFPQE LVRIRSDRDKNLSLRYSVTG P |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human N Cadherin (P19022). |
Additional Info
| Post Translational Modifications | Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa N-terminal soluble fragment and a 45 kDa membrane-bound C-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence. May be phosphorylated by OBSCN. |
| Function | Calcium-dependent cell adhesion protein.preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. Required for proper neurite branching. Required for pre- and postsynaptic organization. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. |
| Protein Name | Cadherin-2Cdw325Neural CadherinN-CadherinCd Antigen Cd325 |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-418990Reactome: R-HSA-525793Reactome: R-HSA-8957275Reactome: R-HSA-9926550 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinSarcolemmaCell JunctionCell SurfaceDesmosomeAdherens JunctionColocalizes With Tmem65 At The Intercalated Disk In CardiomyocytesColocalizes With Obscn At The Intercalated Disk And At Sarcolemma In Cardiomyocytes |
| Alternative Antibody Names | Anti-Cadherin-2 antibodyAnti-Cdw325 antibodyAnti-Neural Cadherin antibodyAnti-N-Cadherin antibodyAnti-Cd Antigen Cd325 antibodyAnti-CDH2 antibodyAnti-CDHN antibodyAnti-NCAD antibody |
Information sourced from Uniprot.org