• Western blot analysis of extracts from wild type (WT) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (STJ11101620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
  • Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (STJ11101620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
  • Immunohistochemistry analysis of N-Cadherin in paraffin-embedded human liver tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of N-Cadherin in paraffin-embedded mouse heart tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of N-Cadherin in paraffin-embedded rat heart tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of N-Cadherin in paraffin-embedded rat liver tissue using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Confocal imaging of paraffin-embedded rat heart using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded mouse heart using [KO Validated] N-Cadherin rabbit monoclonal antibody (STJ11101620, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-CDH2 antibody (100-200) [S0MR] (STJ11101620)

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STJ11101620

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-N-Cadherin (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH2
Gene ID: 1000
Uniprot ID: CADH2_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human N Cadherin (P19022).
Immunogen Sequence: AKFLIYAQDKETQEKWQVAV KLSLKPTLTEESVKESAEVE EIVFPRQFSKHSGHLQRQKR DWVIPPINLPENSRGPFPQE LVRIRSDRDKNLSLRYSVTG P
Post Translational Modifications Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa N-terminal soluble fragment and a 45 kDa membrane-bound C-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence. May be phosphorylated by OBSCN.
Function Calcium-dependent cell adhesion protein.preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. Required for proper neurite branching. Required for pre- and postsynaptic organization. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Protein Name Cadherin-2
Cdw325
Neural Cadherin
N-Cadherin
Cd Antigen Cd325
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-418990
Reactome: R-HSA-525793
Reactome: R-HSA-8957275
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Sarcolemma
Cell Junction
Cell Surface
Desmosome
Adherens Junction
Colocalizes With Tmem65 At The Intercalated Disk In Cardiomyocytes
Colocalizes With Obscn At The Intercalated Disk And At Sarcolemma In Cardiomyocytes
Alternative Antibody Names Anti-Cadherin-2 antibody
Anti-Cdw325 antibody
Anti-Neural Cadherin antibody
Anti-N-Cadherin antibody
Anti-Cd Antigen Cd325 antibody
Anti-CDH2 antibody
Anti-CDHN antibody
Anti-NCAD antibody

Information sourced from Uniprot.org

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