• STJ70446 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70446 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70446 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-CDEP/FARP1 antibody (N-Term) (STJ70446)

SKU:
STJ70446-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human/Mouse/Rat/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CDEP/FARP1 (N-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: FARP1
Gene ID: 10160
Uniprot ID: FARP1_HUMAN
Immunogen Region: N-Term
Accession Number: NP_005757.1; NP_001001715.2; NP_001273768.1
Immunogen Sequence: GEIEQRPTPGSRL
Function Functions as guanine nucleotide exchange factor for RAC1. May play a role in semaphorin signaling. Plays a role in the assembly and disassembly of dendritic filopodia, the formation of dendritic spines, regulation of dendrite length and ultimately the formation of synapses.
Protein Name Ferm - Arhgef And Pleckstrin Domain-Containing Protein 1
Chondrocyte-Derived Ezrin-Like Protein
Ferm - Rhogef And Pleckstrin Domain-Containing Protein 1
Pleckstrin Homology Domain-Containing Family C Member 2
Ph Domain-Containing Family C Member 2
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9035034
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Synapse
Synaptosome
Cytoplasm
Cytosol
Cell Projection
Filopodium
Dendrite
Dendritic Spine
Recruited To The Cell Membrane Via Interaction With Cadm1
Alternative Antibody Names Anti-Ferm - Arhgef And Pleckstrin Domain-Containing Protein 1 antibody
Anti-Chondrocyte-Derived Ezrin-Like Protein antibody
Anti-Ferm - Rhogef And Pleckstrin Domain-Containing Protein 1 antibody
Anti-Pleckstrin Homology Domain-Containing Family C Member 2 antibody
Anti-Ph Domain-Containing Family C Member 2 antibody
Anti-FARP1 antibody
Anti-CDEP antibody
Anti-PLEKHC2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance