• Immunohistochemistry analysis of paraffin-embedded human liver cancer using CDCA3 Rabbit monoclonal antibody (STJ11103582) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of K-562 cells, using CDCA3 Rabbit monoclonal antibody (STJ11103582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDCA3 antibody (1-100) [S2MR] (STJ11103582)

SKU:
STJ11103582

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CDCA3 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDCA3
Gene ID: 83461
Uniprot ID: CDCA3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDCA3 (Q99618).
Immunogen Sequence: MGSAKSVPVTPARPPPHNKH LARVADPRSPSAGILRTPIQ VESSPQPGLPAGEQLEGLKH AQDSDPRSPTLGIARTPMKT SSGDPPSPLVKQLSEVFETE
Post Translational Modifications Ubiquitinated and degraded by the APC/C-Cdh1 complex.
Function F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.
Protein Name Cell Division Cycle-Associated Protein 3
Gene-Rich Cluster Protein C8
Trigger Of Mitotic Entry Protein 1
Tome-1
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Cell Division Cycle-Associated Protein 3 antibody
Anti-Gene-Rich Cluster Protein C8 antibody
Anti-Trigger Of Mitotic Entry Protein 1 antibody
Anti-Tome-1 antibody
Anti-CDCA3 antibody
Anti-C8 antibody
Anti-GRCC8 antibody
Anti-TOME1 antibody

Information sourced from Uniprot.org

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