Anti-CDC42BPB antibody (1641-1690) (STJ94193)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human MRCKB. AA range:1641-1690
General Information
| Short Description | Rabbit polyclonal anti-Serine/threonine-protein kinase MRCK beta (1641-1690) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDC42BPB |
| Gene ID | 9578 |
| Uniprot ID | MRCKB_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRCKB. AA range:1641-1690 |
| Immunogen Region | 1641-1690 |
| Specificity | MRCK Beta Polyclonal Antibody detects endogenous levels of MRCK Beta protein. |
Additional Info
| Tissue Specificity | Expressed in all tissues examined, with high levels in heart, brain, placenta and lung. |
| Post Translational Modifications | Proteolytically cleaved by caspases upon apoptosis induction. |
| Function | Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation. |
| Protein Name | Serine/Threonine-Protein Kinase Mrck BetaCdc42-Binding Protein Kinase BetaCdc42bp-BetaDmpk-Like BetaMyotonic Dystrophy Kinase-Related Cdc42-Binding Kinase BetaMrck BetaMyotonic Dystrophy Protein Kinase-Like Beta |
| Database Links | Reactome: R-HSA-9013148Reactome: R-HSA-9013406Reactome: R-HSA-9013409 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionCell ProjectionLamellipodiumDisplays A Dispersed Punctate Distribution And Concentrates Along The Cell PeripheryEspecially At The Leading Edge And Cell-Cell JunctionThis Concentration Is Ph-Domain DependentDetected At The Leading Edge Of Migrating CellsLocalization At The Leading Edge Of Migrating Cells Requires Interaction With Catalytically Active Cdc42Localizes In The Lamellipodium In A Fam89b/Lrap25-Dependent Manner |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Mrck Beta antibodyAnti-Cdc42-Binding Protein Kinase Beta antibodyAnti-Cdc42bp-Beta antibodyAnti-Dmpk-Like Beta antibodyAnti-Myotonic Dystrophy Kinase-Related Cdc42-Binding Kinase Beta antibodyAnti-Mrck Beta antibodyAnti-Myotonic Dystrophy Protein Kinase-Like Beta antibodyAnti-CDC42BPB antibodyAnti-KIAA1124 antibody |
Information sourced from Uniprot.org