| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CDC25A (86-328) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CDC25A |
| Gene ID: |
993 |
| Uniprot ID: |
MPIP1_HUMAN |
| Immunogen Region: |
86-328 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 86-328 of CDC25A (NP_001780.2). |
| Immunogen Sequence: |
LDSPGPLDSKENLENPMRRI HSLPQKLLGCSPALKRSHSD SLDHDIFQLIDPDENKENEA FEFKKPVRPVSRGCLHSHGL QEGKDLFTQRQNSAPARMLS SNERDSSEPGNFIPLFTPQS PVTATLSDEDDGFVDLLDGE NLKNEEETPSCMASLWTAPL VMRTTNLDNRCKLFDSPSLC SSSTRSVLKRPERSQEESPP GSTKRRKSMSGASPKESTNP EKAHETLHQSLSLASSPKG |
| Post Translational Modifications | Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization. |
| Function | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. |
| Protein Name | M-Phase Inducer Phosphatase 1Dual Specificity Phosphatase Cdc25a |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-156711Reactome: R-HSA-176187Reactome: R-HSA-5689880Reactome: R-HSA-69202Reactome: R-HSA-69273Reactome: R-HSA-69601Reactome: R-HSA-69656Reactome: R-HSA-8862803 |
| Alternative Antibody Names | Anti-M-Phase Inducer Phosphatase 1 antibodyAnti-Dual Specificity Phosphatase Cdc25a antibodyAnti-CDC25A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
