Anti-CDC20 antibody (1-100 aa) [SRM5262] (STJ11107164)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal CDC20 (1-100 aa) antibody for use in WB, IP and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5262 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDC20 |
| Gene ID | 991 |
| Uniprot ID | CDC20_HUMAN |
| Immunogen Region | 1-100 aa |
| Immunogen Sequence | MAQFAFESDLHSLLQLDAPI PNAPPARWQRKAKEAAGPAP SPMRAANRSHSAGRTPGRTP GKSSSKVQTTPSKPGGDRYI PHRSAAQMEVASFLLSKENQ |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDC20 (NP_001246.2๏ปฟ). |
Additional Info
| Post Translational Modifications | Acetylated. Deacetylated at Lys-66 by SIRT2.deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C). Phosphorylated during mitosis. Phosphorylated by BUB1 at Ser-41.Ser-72.Ser-92.Ser-153.Thr-157 and Ser-161. Phosphorylated by NEK2. Dephosphorylated by CTDP1. Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex. Ubiquitinated by UBR5 when not assembled in a multiprotein complex, leading to its degradation: UBR5 recognizes and binds a degron that is not accessible when CDC20 is part of a complex. |
| Function | Substrate-specific adapter of the anaphase promoting complex/cyclosome (APC/C) complex that confers substrate specificity by binding to substrates and targeting them to the APC/C complex for ubiquitination and degradation. Recognizes and binds the destruction box (D box) on protein substrates. Involved in the metaphase/anaphase transition of cell cycle. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41. |
| Protein Name | Cell Division Cycle Protein 20 HomologP55cdc |
| Database Links | Reactome: R-HSA-141405Reactome: R-HSA-141430Reactome: R-HSA-141444Reactome: R-HSA-174048Reactome: R-HSA-174113Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176409Reactome: R-HSA-176417Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-5689880Reactome: R-HSA-68877Reactome: R-HSA-9648025Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeChromosomeCentromereKinetochoreSpindle Pole |
| Alternative Antibody Names | Anti-Cell Division Cycle Protein 20 Homolog antibodyAnti-P55cdc antibodyAnti-CDC20 antibody |
Information sourced from Uniprot.org