Anti-CD8b antibody [EBS-CD-007] (STJ16101131)

SKU:
STJ16101131

£274.50 - £1,323.50
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Host: Mouse
Applications: FC/IF/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-CD8b is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: EBS-CD-007
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.02% Sodium Azide.
Purification: Affinity purified from tissue culture.
Concentration: Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range: Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate antigen retrieval method for staining of formalin-fixed tissues has not been established to date
Storage Instruction: Store for up to 1 year at 2-8°C upon receipt.
Gene Symbol: CD8B
Gene ID: 926
Uniprot ID: CD8B_HUMAN
Immunogen: A BALB/c mouse was immunized with human PBL. Fusion partner: NS-1.
Tissue Specificity Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
Post Translational Modifications Phosphorylated as a consequence of T-cell activation. Palmitoylated at the cytoplasmic tail and thereby targets the heterodimer CD8A/CD8B to lipid rafts unlike CD8A homodimers.
Function Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells.
Protein Name T-Cell Surface Glycoprotein Cd8 Beta Chain
Cd Antigen Cd8b
Database Links Reactome: R-HSA-182218
Reactome: R-HSA-198933
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Requires The Partner Cd8a For Efficient Cell Surface Expression
The Heterodimer Cd8a/Cd8b Localizes To Lipid Rafts Due To Cd8b Cytoplasmic Tail Palmitoylation
Isoform 2: Cell Membrane
Isoform 3: Secreted
Isoform 4: Cell Membrane
Isoform 5: Cell Membrane
Isoform 6: Secreted
Isoform 7: Secreted
Isoform 8: Secreted
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd8 Beta Chain antibody
Anti-Cd Antigen Cd8b antibody
Anti-CD8B antibody
Anti-CD8B1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance