• Western blot analysis of various lysates, using CD8A Rabbit polyclonal antibody (STJ11104719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD8A antibody (STJ11104719)

SKU:
STJ11104719

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD8A is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Cd8a
Gene ID: 12525
Uniprot ID: CD8A_MOUSE
Immunogen: Recombinant protein of mouse CD8A
Post Translational Modifications Palmitoylated, but association with CD8B seems to be more important for the enrichment of CdD8A in lipid rafts. Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation.
Function Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Protein Name T-Cell Surface Glycoprotein Cd8 Alpha Chain
T-Cell Surface Glycoprotein Lyt-2
Cd Antigen Cd8a
Database Links Reactome: R-MMU-198933
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cd8a Localizes To Lipid Rafts Only When Associated With Its Partner Cd8b
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd8 Alpha Chain antibody
Anti-T-Cell Surface Glycoprotein Lyt-2 antibody
Anti-Cd Antigen Cd8a antibody
Anti-Cd8a antibody
Anti-Lyt-2 antibody
Anti-Lyt2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance