Anti-Cd8a antibody [OX-8] {PE/Cyanine5.5} (STJA0001627)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationPE/Cyanine5.5
IsotypeIgG1k
General Information
| Short Description | Mouse monoclonal anti-T-cell surface glycoprotein CD8 alpha chain for use in FC in Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC |
| Host | Mouse |
| Reactivity | Rat |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | OX-8 |
| Isotype | IgG1k |
| Conjugation | PE/Cyanine5.5 |
| Concentration | 5 Mu L/Test |
| Formulation | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
| Storage Instruction | Store between 2-8°C and protecting from prolonged exposure to light. Do not freeze this product. |
Target Information
| Gene Symbol | Cd8a |
| Gene ID | 24930 |
| Uniprot ID | CD8A_RAT |
Additional Info
| Post Translational Modifications | Palmitoylated, but association with CD8B seems to be more important for the enrichment of CD8A in lipid rafts. O-glycosylated. Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. |
| Protein Name | T-Cell Surface Glycoprotein Cd8 Alpha ChainCd8 Antigen 32 Kda ChainOx-8 Membrane AntigenCd Antigen Cd8a |
| Database Links | Reactome: R-RNO-198933 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCd8a Localizes To Lipid Rafts Only When Associated With Its Partner Cd8b |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd8 Alpha Chain antibodyAnti-Cd8 Antigen 32 Kda Chain antibodyAnti-Ox-8 Membrane Antigen antibodyAnti-Cd Antigen Cd8a antibodyAnti-Cd8a antibody |
Information sourced from Uniprot.org