• Immunofluorescence analysis of HeLa cells using CD84 antibody (STJ28516). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using CD84 Rabbit polyclonal antibody (STJ28516) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD84 antibody (22-225) (STJ28516)

SKU:
STJ28516

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD84 (22-225) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD84
Gene ID: 8832
Uniprot ID: SLAF5_HUMAN
Immunogen Region: 22-225
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-225 of human CD84 (NP_003865.1).
Immunogen Sequence: KDSEIFTVNGILGESVTFPV NIQEPRQVKIIAWTSKTSVA YVTPGDSETAPVVTVTHRNY YERIHALGPNYNLVISDLRM EDAGDYKADINTQADPYTTT KRYNLQIYRRLGKPKITQSL MASVNSTCNVTLTCSVEKEE KNVTYNWSPLGEEGNVLQIF QTPEDQELTYTCTAQNPVSN NSDSISARQLCADIAMGFRT HHTG
Tissue Specificity Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells. Expressed in mast cells.
Post Translational Modifications Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A is dependent of tyrosine phosphorylation of its cytoplasmic domain. Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation. Phosphorylated on tyrosine residues upon high affinity immunoglobulin epsilon receptor aggregation in mast cells. N-glycosylated.
Function Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seem be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. May serve as a marker for hematopoietic progenitor cells Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation. In germinal centers involved in maintaining B-cell tolerance and in preventing autoimmunity. In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling.independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1. In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion.involving ITSM 2. Positively regulates macroautophagy in primary dendritic cells via stabilization of IRF8.inhibits TRIM21-mediated proteasomal degradation of IRF8.
Protein Name Slam Family Member 5
Cell Surface Antigen Max.3
Hly9-Beta
Leukocyte Differentiation Antigen Cd84
Signaling Lymphocytic Activation Molecule 5
Cd Antigen Cd84
Database Links Reactome: R-HSA-202733
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Slam Family Member 5 antibody
Anti-Cell Surface Antigen Max.3 antibody
Anti-Hly9-Beta antibody
Anti-Leukocyte Differentiation Antigen Cd84 antibody
Anti-Signaling Lymphocytic Activation Molecule 5 antibody
Anti-Cd Antigen Cd84 antibody
Anti-CD84 antibody
Anti-SLAMF5 antibody

Information sourced from Uniprot.org

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