Anti-CD84 antibody (22-225) (STJ28516)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CD84 (22-225) antibody for use in WB, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CD84 |
| Gene ID | 8832 |
| Uniprot ID | SLAF5_HUMAN |
| Immunogen Region | 22-225 |
| Immunogen Sequence | KDSEIFTVNGILGESVTFPV NIQEPRQVKIIAWTSKTSVA YVTPGDSETAPVVTVTHRNY YERIHALGPNYNLVISDLRM EDAGDYKADINTQADPYTTT KRYNLQIYRRLGKPKITQSL MASVNSTCNVTLTCSVEKEE KNVTYNWSPLGEEGNVLQIF QTPEDQELTYTCTAQNPVSN NSDSISARQLCADIAMGFRT HHTG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 22-225 of human CD84 (NP_003865.1). |
Additional Info
| Tissue Specificity | Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells. Expressed in mast cells. |
| Post Translational Modifications | Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A is dependent of tyrosine phosphorylation of its cytoplasmic domain. Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation. Phosphorylated on tyrosine residues upon high affinity immunoglobulin epsilon receptor aggregation in mast cells. N-glycosylated. |
| Function | Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seem be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. May serve as a marker for hematopoietic progenitor cells Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation. In germinal centers involved in maintaining B-cell tolerance and in preventing autoimmunity. In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling.independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1. In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion.involving ITSM 2. Positively regulates macroautophagy in primary dendritic cells via stabilization of IRF8.inhibits TRIM21-mediated proteasomal degradation of IRF8. |
| Protein Name | Slam Family Member 5Cell Surface Antigen Max.3Hly9-BetaLeukocyte Differentiation Antigen Cd84Signaling Lymphocytic Activation Molecule 5Cd Antigen Cd84 |
| Database Links | Reactome: R-HSA-202733 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Slam Family Member 5 antibodyAnti-Cell Surface Antigen Max.3 antibodyAnti-Hly9-Beta antibodyAnti-Leukocyte Differentiation Antigen Cd84 antibodyAnti-Signaling Lymphocytic Activation Molecule 5 antibodyAnti-Cd Antigen Cd84 antibodyAnti-CD84 antibodyAnti-SLAMF5 antibody |
Information sourced from Uniprot.org