• Western blot analysis of extracts of various cell lines, using CD82 rabbit monoclonal antibody (STJ11102466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Confocal imaging of paraffin-embedded Human breast cancer using CD82 Rabbit monoclonal antibody (STJ11102466, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry of paraffin-embedded human colon using CD82 rabbit monoclonal antibody (STJ11102466) at dilution of 1:100 (40x lens).
  • Confocal imaging of Raji cells using CD82 Rabbit monoclonal antibody (STJ11102466, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of Jurkat cells using CD82 rabbit monoclonal antibody (STJ11102466) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal imaging of RAW 264. 7 cells using CD82 Rabbit monoclonal antibody (STJ11102466, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of Raw264 7 cells using CD82 rabbit monoclonal antibody (STJ11102466) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CD82 in paraffin-embedded human colon using CD82 Rabbit monoclonal antibody (STJ11102466) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using CD82 Rabbit monoclonal antibody (STJ11102466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD82 antibody (168-267) [S6MR] (STJ11102466)

SKU:
STJ11102466

€183.13 - €799.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD82 (168-267) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD82
Gene ID: 3732
Uniprot ID: CD82_HUMAN
Immunogen Region: 168-267
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 168-267 of human CD82 (P27701).
Immunogen Sequence: PEVTYPCSCEVKGEEDNSLS VRKGFCEAPGNRTQSGNHPE DWPVYQEGCMEKVQAWLQEN LGIILGVGVGVAIIELLGMV LSICLCRHVHSEDYSKVPKY
Tissue Specificity Lymphoid specific.
Function Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Protein Name Cd82 Antigen
C33 Antigen
Ia4
Inducible Membrane Protein R2
Metastasis Suppressor Kangai-1
Suppressor Of Tumorigenicity 6 Protein
Tetraspanin-27
Tspan-27
Cd Antigen Cd82
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Cd82 Antigen antibody
Anti-C33 Antigen antibody
Anti-Ia4 antibody
Anti-Inducible Membrane Protein R2 antibody
Anti-Metastasis Suppressor Kangai-1 antibody
Anti-Suppressor Of Tumorigenicity 6 Protein antibody
Anti-Tetraspanin-27 antibody
Anti-Tspan-27 antibody
Anti-Cd Antigen Cd82 antibody
Anti-CD82 antibody
Anti-KAI1 antibody
Anti-SAR2 antibody
Anti-ST6 antibody
Anti-TSPAN27 antibody

Information sourced from Uniprot.org

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