• Western blot analysis of lysate from 4T1 cells, using CD81 Antibody.
  • Western blot analysis of 4T1 cells using CD81 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000
  • Immunofluorescence analysis of mouse-kidney tissue. 1, CD81 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-spleen tissue. 1, CD81 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-CD81 antibody (111-160 aa) (STJ96759)

SKU:
STJ96759

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD81 antigen (111-160 aa) is suitable for use in Immunofluorescence, Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD81
Gene ID: 975
Uniprot ID: CD81_HUMAN
Immunogen Region: 111-160 aa
Specificity: CD81 Polyclonal Antibody detects endogenous levels of CD81 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human CD81 at the amino acid range 111-160
Post Translational Modifications Not glycosylated. Likely constitutively palmitoylated at low levels. Protein palmitoylation is up-regulated upon coligation of BCR and CD9-C2R-CD81 complexes in lipid rafts.
Function Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells. Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production. In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype. Present in MHC class II compartments, may also play a role in antigen presentation. Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. Positively regulates sperm-egg fusion and may be involved in acrosome reaction. In myoblasts, associates with CD9 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion of mononuclear cell progenitors into osteoclasts in charge of bone resorption. May regulate the compartmentalization of enzymatic activities. In T cells, defines the subcellular localization of dNTPase SAMHD1 and permits its degradation by the proteasome, thereby controlling intracellular dNTP levels. Also involved in cell adhesion and motility. Positively regulates integrin-mediated adhesion of macrophages, particularly relevant for the inflammatory response in the lung. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Association with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. (Microbial infection) Involved in SAMHD1-dependent restriction of HIV-1 replication. May support early replication of both R5- and X4-tropic HIV-1 viruses in T cells, likely via proteasome-dependent degradation of SAMHD1. (Microbial infection) Specifically required for Plasmodium falciparum infectivity of hepatocytes, controlling sporozoite entry into hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms.
Protein Name Cd81 Antigen
26 Kda Cell Surface Protein Tapa-1
Target Of The Antiproliferative Antibody 1
Tetraspanin-28
Tspan-28
Cd Antigen Cd81
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-977606
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Associates With Cldn1 And The Cldn1-Cd81 Complex Localizes To The Basolateral Cell Membrane
Alternative Antibody Names Anti-Cd81 Antigen antibody
Anti-26 Kda Cell Surface Protein Tapa-1 antibody
Anti-Target Of The Antiproliferative Antibody 1 antibody
Anti-Tetraspanin-28 antibody
Anti-Tspan-28 antibody
Anti-Cd Antigen Cd81 antibody
Anti-CD81 antibody
Anti-TAPA1 antibody
Anti-TSPAN28 antibody

Information sourced from Uniprot.org

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