• Western blot analysis of various lysates, using CD79B rabbit monoclonal antibody (STJ11103563) at 1:9000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded Human brain using CD79B rabbit monoclonal antibody (STJ11103563) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD79B rabbit monoclonal antibody (STJ11103563) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD79B rabbit monoclonal antibody (STJ11103563) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of paraffin-embedded Human spleen using CD79B rabbit monoclonal antibody (STJ11103563, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded mouse spleen using CD79B rabbit monoclonal antibody (STJ11103563, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded rat spleen using CD79B rabbit monoclonal antibody (STJ11103563, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-CD79B antibody (1-100) [S3MR] (STJ11103563)

SKU:
STJ11103563

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD79B (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
IHC-P 1:50-1:200
IF/ICC 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD79B
Gene ID: 974
Uniprot ID: CD79B_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD79B (P40259).
Immunogen Sequence: MARLALSPVPSHWMVALLLL LSAEPVPAARSEDRYRNPKG SACSRIWQSPRFIARKRGFT VKMHCYMNSASGNVSWLWKQ EMDENPQQLKLEKGRMEESQ
Tissue Specificity B-cells.
Post Translational Modifications Phosphorylated on tyrosine upon B-cell activation by SRC-type Tyr-kinases such as BLK, LYN and SYK.
Function Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
Protein Name B-Cell Antigen Receptor Complex-Associated Protein Beta Chain
B-Cell-Specific Glycoprotein B29
Ig-Beta
Immunoglobulin-Associated B29 Protein
Cd Antigen Cd79b
Database Links Reactome: R-HSA-5690714
Reactome: R-HSA-9679191
Reactome: R-HSA-983695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Following Antigen Binding
The Bcr Has Been Shown To Translocate From Detergent-Soluble Regions Of The Cell Membrane To Lipid Rafts Although Signal Transduction Through The Complex Can Also Occur Outside Lipid Rafts
Alternative Antibody Names Anti-B-Cell Antigen Receptor Complex-Associated Protein Beta Chain antibody
Anti-B-Cell-Specific Glycoprotein B29 antibody
Anti-Ig-Beta antibody
Anti-Immunoglobulin-Associated B29 Protein antibody
Anti-Cd Antigen Cd79b antibody
Anti-CD79B antibody
Anti-B29 antibody
Anti-IGB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance