Anti-CD74 antibody (150-250) [ABT202] (STJA0038972)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
ImmunogenSynthesized peptide derived from human CD74 AA range: 150-250
General Information
| Short Description | Mouse monoclonal anti-Hla Class Ii Histocompatibility antigen Gamma Chain (150-250) for use in IHC, WB, IF and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB/IF/ELISA |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ABT202 |
| Isotype | IgG2ak |
| Conjugation | Unconjugated |
| Purification | Protein G |
| Dilution Range | IHC 1:200-1000WB 1:500-2000IF 1:100-500ELISA 1:1000-5000 |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CD74 |
| Gene ID | 972 |
| Uniprot ID | HG2A_HUMAN |
| Immunogen | Synthesized peptide derived from human CD74 AA range: 150-250 |
| Immunogen Region | 150-250 |
| Specificity | This antibody detects endogenous levels of CD74 protein. |
Additional Info
| Post Translational Modifications | O-glycosylated with core 1 or possibly core 8 glycans. Contains chondroitin sulfate. |
| Function | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. Class-II-associated invariant chain peptide: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome. Isoform p41: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2. Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform. |
| Protein Name | Hla Class Ii Histocompatibility Antigen Gamma ChainHla-Dr Antigens-Associated Invariant ChainIa Antigen-Associated Invariant ChainIiCd Antigen Cd74 Cleaved Into - Class-Ii-Associated Invariant Chain PeptideClip |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-2132295 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinEndoplasmic Reticulum MembraneGolgi ApparatusTrans-Golgi NetworkEndosomeLysosomeSecretedTransits Through A Number Of Intracellular Compartments In The Endocytic PathwayIt Can Either Undergo Proteolysis Or Reach The Cell MembraneIsoform P41: Late Endosome |
| Alternative Antibody Names | Anti-Hla Class Ii Histocompatibility Antigen Gamma Chain antibodyAnti-Hla-Dr Antigens-Associated Invariant Chain antibodyAnti-Ia Antigen-Associated Invariant Chain antibodyAnti-Ii antibodyAnti-Cd Antigen Cd74 Cleaved Into - Class-Ii-Associated Invariant Chain Peptide antibodyAnti-Clip antibodyAnti-CD74 antibodyAnti-DHLAG antibody |
Information sourced from Uniprot.org