• Western blot analysis of extracts of various cell lines, using CD74 rabbit monoclonal antibody (STJ11102439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CD74 rabbit monoclonal antibody (STJ11102439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human small cell lung cancer using CD74 rabbit monoclonal antibody (STJ11102439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD74 rabbit monoclonal antibody (STJ11102439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD74 antibody (1-100) [S9MR] (STJ11102439)

SKU:
STJ11102439

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD74 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD74
Gene ID: 972
Uniprot ID: HG2A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD74 (P04233).
Immunogen Sequence: MHRRRSRSCREDQKPVMDDQ RDLISNNEQLPMLGRRPGAP ESKCSRGALYTGFSILVTLL LAGQATTAYFLYQQQGRLDK LTVTSQNLQLENLRMKLPKP
Tissue Specificity Detected in urine (at protein level). Isoform p41: In B cells, represents 10% of total CD74 expression. Isoform p33: In B cells, represents 70% of total CD74 expression.
Post Translational Modifications O-glycosylated with core 1 or possibly core 8 glycans. Contains chondroitin sulfate.
Function Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. Class-II-associated invariant chain peptide: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome. Isoform p41: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2. Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform.
Protein Name Hla Class Ii Histocompatibility Antigen Gamma Chain
Hla-Dr Antigens-Associated Invariant Chain
Ia Antigen-Associated Invariant Chain
Ii
Cd Antigen Cd74 Cleaved Into - Class-Ii-Associated Invariant Chain Peptide
Clip
Database Links Reactome: R-HSA-202733
Reactome: R-HSA-2132295
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Endoplasmic Reticulum Membrane
Golgi Apparatus
Trans-Golgi Network
Endosome
Lysosome
Secreted
Transits Through A Number Of Intracellular Compartments In The Endocytic Pathway
It Can Either Undergo Proteolysis Or Reach The Cell Membrane
Isoform P41: Late Endosome
Alternative Antibody Names Anti-Hla Class Ii Histocompatibility Antigen Gamma Chain antibody
Anti-Hla-Dr Antigens-Associated Invariant Chain antibody
Anti-Ia Antigen-Associated Invariant Chain antibody
Anti-Ii antibody
Anti-Cd Antigen Cd74 Cleaved Into - Class-Ii-Associated Invariant Chain Peptide antibody
Anti-Clip antibody
Anti-CD74 antibody
Anti-DHLAG antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance