| Short Description : | Mouse monoclonal anti-CD73/NT5E for use in ELISA, WB and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | ELISA/WB/IP |
| Host: | Mouse |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | M064 |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Purification: | Protein G Purified |
| Dilution Range: | WB 1:500IP 1:50 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction: | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: | NT5E |
| Gene ID: | 4907 |
| Uniprot ID: | 5NTD_HUMAN |
| Immunogen: | Clone (M064) was generated from a proprietary antigen related to the mature form of CD73 expressed in the NCI-H1299 lung cancer cell line. |
| Immunogen Sequence: | Human |
| Specificity: | Clone M064 mouse monoclonal antibody detects a 65 kDa* protein on SDSPAGE Native" immunoblots of human H1299, H1915, and MDA-MB-231 carcinomas. This antibody weakly detects denatured CD73. The antibody works for native western blot, immunoprecipitati |
| Function | Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. |
| Protein Name | 5'-Nucleotidase5'-Nt 5'-DeoxynucleotidaseEcto-5'-NucleotidaseImp-Specific 5'-NucleotidaseThymidylate 5'-PhosphataseCd Antigen Cd73 |
| Database Links | Reactome: R-HSA-196807Reactome: R-HSA-73621Reactome: R-HSA-74259Reactome: R-HSA-9660826 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative Antibody Names | Anti-5'-Nucleotidase antibodyAnti-5'-Nt antibodyAnti-5'-Deoxynucleotidase antibodyAnti-Ecto-5'-Nucleotidase antibodyAnti-Imp-Specific 5'-Nucleotidase antibodyAnti-Thymidylate 5'-Phosphatase antibodyAnti-Cd Antigen Cd73 antibodyAnti-NT5E antibodyAnti-NT5 antibodyAnti-NTE antibody |
Information sourced from Uniprot.org
