![Anti-CD66e/CEA/CEACAM5 nanobody [SAA0838] (STJN000019)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/200632/412814/STJN000019-SDS-PAGE-1__69899.1749232368.jpg?c=1 "Anti-CD66e/CEA/CEACAM5 nanobody [SAA0838] (STJN000019)")
![Anti-CD66e/CEA/CEACAM5 nanobody [SAA0838] (STJN000019)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/200632/412814/STJN000019-SDS-PAGE-1__69899.1749232368.jpg?c=1 "Anti-CD66e/CEA/CEACAM5 nanobody [SAA0838] (STJN000019)")
Anti-CD66e/CEA/CEACAM5 nanobody [SAA0838] (STJN000019)
General Information
| Short Description | Alpaca monoclonal nanobody anti-Cell Adhesion Molecule Ceacam5 is suitable for use in ELISA, Flow Cytometry and SPR research applications. |
| Applications | ELISA/FC/SPR |
| Host | Alpaca |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA0838 |
| Isotype | VHH-8His-Cys-tag |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by Nickel column. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4Β°C short term (1-2 weeks). Store at-20Β°C 12 months. Store at-80Β°C long term. |
Target Information
| Gene Symbol | CEACAM5 |
| Gene ID | 1048 |
| Uniprot ID | CEAM5_HUMAN |
Additional Info
| Post Translational Modifications | Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. |
| Function | Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression.induces resistance to anoikis of colorectal carcinoma cells. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. |
| Protein Name | Cell Adhesion Molecule Ceacam5Carcinoembryonic AntigenCeaCarcinoembryonic Antigen-Related Cell Adhesion Molecule 5Cea Cell Adhesion Molecule 5Meconium Antigen 100Cd Antigen Cd66e |
| Database Links | Reactome: R-HSA-163125Reactome: R-HSA-202733 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorApical Cell MembraneCell SurfaceLocalized To The Apical Glycocalyx Surface |
| Alternative Nanobody Names | Anti-Cell Adhesion Molecule Ceacam5 nanobodyAnti-Carcinoembryonic Antigen nanobodyAnti-Cea nanobodyAnti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 nanobodyAnti-Cea Cell Adhesion Molecule 5 nanobodyAnti-Meconium Antigen 100 nanobodyAnti-Cd Antigen Cd66e nanobodyAnti-CEACAM5 nanobodyAnti-CEA nanobody |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Anti-CD66e/CEA/CEACAM5 nanobody [SAA0838] (STJN000019)
This nanobody binds CD66e, also known as CEA or CEACAM5 (UniProt P06731), and has confirmed reactivity against the human form of the antigen. No cross-reactivity with other species has been documented in the provided product data.
This nanobody has been validated for use in ELISA, flow cytometry (FC), and surface plasmon resonance (SPR). Researchers should optimize conditions for their specific experimental setup, as performance may vary by protocol.
This nanobody is supplied in an unconjugated VHH format carrying a C-terminal 8His-Cys tag, which supports nickel-based detection, site-specific conjugation, and immobilization strategies. It is provided at a concentration of 1 mg/mL in 0.01M PBS, pH 7.4.
This nanobody is purified by nickel affinity column chromatography and has a confirmed purity of greater than 95% as determined by SDS-PAGE.
For short-term storage of 1 to 2 weeks, keep at 4Β°C. For storage up to 12 months, store at -20Β°C, and for long-term storage beyond that, use -80Β°C. A manual defrost freezer is recommended, and repeated freeze-thaw cycles should be avoided.
No dissociation constant or affinity constant data is listed in the current product specification. Researchers requiring quantitative binding kinetics data may wish to contact St John's Laboratory directly or conduct their own SPR characterization, as this nanobody has been validated for SPR applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.