![Anti-CD66e/CEA/CEACAM5 nanobody [SAA0837] (STJN000018)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/200631/412813/STJN000018-SDS-PAGE-1__08438.1749232367.jpg?c=1 "Anti-CD66e/CEA/CEACAM5 nanobody [SAA0837] (STJN000018)")
![Anti-CD66e/CEA/CEACAM5 nanobody [SAA0837] (STJN000018)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/200631/412813/STJN000018-SDS-PAGE-1__08438.1749232367.jpg?c=1 "Anti-CD66e/CEA/CEACAM5 nanobody [SAA0837] (STJN000018)")
Anti-CD66e/CEA/CEACAM5 nanobody [SAA0837] (STJN000018)
General Information
| Short Description | Alpaca monoclonal nanobody anti-Cell Adhesion Molecule Ceacam5 is suitable for use in ELISA, Flow Cytometry and SPR research applications. |
| Applications | ELISA/FC/SPR |
| Host | Alpaca |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA0837 |
| Isotype | VHH-8His-Cys-tag |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by Nickel column. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at-20°C 12 months. Store at-80°C long term. |
Target Information
| Gene Symbol | CEACAM5 |
| Gene ID | 1048 |
| Uniprot ID | CEAM5_HUMAN |
Additional Info
| Post Translational Modifications | Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. |
| Function | Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression.induces resistance to anoikis of colorectal carcinoma cells. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. |
| Protein Name | Cell Adhesion Molecule Ceacam5Carcinoembryonic AntigenCeaCarcinoembryonic Antigen-Related Cell Adhesion Molecule 5Cea Cell Adhesion Molecule 5Meconium Antigen 100Cd Antigen Cd66e |
| Database Links | Reactome: R-HSA-163125Reactome: R-HSA-202733 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorApical Cell MembraneCell SurfaceLocalized To The Apical Glycocalyx Surface |
| Alternative Nanobody Names | Anti-Cell Adhesion Molecule Ceacam5 nanobodyAnti-Carcinoembryonic Antigen nanobodyAnti-Cea nanobodyAnti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 nanobodyAnti-Cea Cell Adhesion Molecule 5 nanobodyAnti-Meconium Antigen 100 nanobodyAnti-Cd Antigen Cd66e nanobodyAnti-CEACAM5 nanobodyAnti-CEA nanobody |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Anti-CD66e/CEA/CEACAM5 nanobody [SAA0837] (STJN000018)
This nanobody targets CD66e/CEA/CEACAM5 (UniProt P06731) and has confirmed reactivity against the human form of the protein. No cross-reactivity with other species has been documented in the provided product data.
This nanobody has been validated for use in ELISA, flow cytometry (FC), and surface plasmon resonance (SPR). Researchers should perform application-specific optimization for their own experimental conditions.
This nanobody is supplied in VHH format with a C-terminal 8His-Cys tag and is unconjugated. The His tag enables detection or capture via standard anti-His reagents, and the Cys tag supports site-specific conjugation if required.
Purity is greater than 95% as determined by SDS-PAGE. The nanobody is purified by nickel-affinity column chromatography, consistent with its 8His tag format.
This nanobody is supplied at 1 mg/mL in 0.01M PBS, pH 7.4. It should be stored at 4°C for short-term use of 1 to 2 weeks, at -20°C for up to 12 months, or at -80°C for long-term storage. Repeated freeze-thaw cycles should be avoided, and a manual defrost freezer is recommended.
No dissociation constant or affinity constant data is included in the current product specifications. Binding characterization via SPR is listed as a validated application, so researchers can use this nanobody to generate kinetic data in their own assay setups.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.