| Host: |
Mouse |
| Applications: |
WB/ICC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-CD63 (Extracellular region) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M040 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2a |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Protein G Purified |
| Dilution Range: |
WB 1:100IHCICC 1:500IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CD63 |
| Gene ID: |
967 |
| Uniprot ID: |
CD63_HUMAN |
| Immunogen Region: |
Extracellular region |
| Specificity: |
Clone M040 mouse monoclonal antibody has high reactivity to CD63 at the plasma membrane in immunocytochemical assays, and weakly detects a 30-60 kDa* protein on SDS-PAGE Native" immunoblots of human A431, MeWo, and A549 cells. The antibody does not d |
| Immunogen: |
Clone (M040) was generated from a proprietary antigen related to the native human CD63 expressed in MeWo melanoma cell line. |
| Tissue Specificity | Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. |
| Post Translational Modifications | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). |
| Function | Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. |
| Protein Name | Cd63 AntigenGranulophysinLysosomal-Associated Membrane Protein 3Lamp-3Lysosome Integral Membrane Protein 1Limp1Melanoma-Associated Antigen Me491Oma81hOcular Melanoma-Associated AntigenTetraspanin-30Tspan-30Cd Antigen Cd63 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinLysosome MembraneLate Endosome MembraneEndosomeMultivesicular BodyMelanosomeSecretedExtracellular ExosomeCell SurfaceAlso Found In Weibel-Palade Bodies Of Endothelial CellsLocated In Platelet Dense GranulesDetected In A Subset Of Pre-MelanosomesDetected On Intralumenal Vesicles (Ilvs) Within Multivesicular Bodies |
| Alternative Antibody Names | Anti-Cd63 Antigen antibodyAnti-Granulophysin antibodyAnti-Lysosomal-Associated Membrane Protein 3 antibodyAnti-Lamp-3 antibodyAnti-Lysosome Integral Membrane Protein 1 antibodyAnti-Limp1 antibodyAnti-Melanoma-Associated Antigen Me491 antibodyAnti-Oma81h antibodyAnti-Ocular Melanoma-Associated Antigen antibodyAnti-Tetraspanin-30 antibodyAnti-Tspan-30 antibodyAnti-Cd Antigen Cd63 antibodyAnti-CD63 antibodyAnti-MLA1 antibodyAnti-TSPAN30 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
