Anti-CD61 antibody [EBS-CD-034] (STJ16101119)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgMk
ImmunogenA BALB/c mouse was immunized with stimulated human PBMC s. Fusion partner: NS-1.
General Information
| Short Description | Mouse monoclonal anti-CD61 for use in FC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/IHC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | EBS-CD-034 |
| Isotype | IgMk |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate antigen retrieval method for staining of formalin-fixed tissues has not been established to date |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Immunogen | A BALB/c mouse was immunized with stimulated human PBMC s. Fusion partner: NS-1. |
Additional Info
| Background | EBS-CD-034 reacts with a complex of CD41 and CD61, i.e. alpha IIb integrin and the integrin beta chain. The MW of the GPIIIa is 105 kDa unreduced and 90 kDa reduced. The integrin beta 3 chain can also form a complex called the vitronectin receptor with integrin alpha V: the CD51/CD61 complex. Ligands are fibrinogen, fibronectin, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The CD51/CD61 complex is also found on endothelial cells, some B-cells, monocytes/macrophages and tumor cells. |
Information sourced from Uniprot.org