• Immunohistochemistry analysis of paraffin-embedded mouse testis using CD59 Rabbit monoclonal antibody (STJ11101994) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using CD59 Rabbit monoclonal antibody (STJ11101994) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CD59 Rabbit monoclonal antibody (STJ11101994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD59 antibody (49-128) [S4MR] (STJ11101994)

SKU:
STJ11101994

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD59 (49-128) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD59
Gene ID: 966
Uniprot ID: CD59_HUMAN
Immunogen Region: 49-128
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 49-128 of human CD59 (NP_000602.1).
Immunogen Sequence: DACLITKAGLQVYNKCWKFE HCNFNDVTTRLRENELTYYC CKKDLCNFNEQLENGGTSLS EKTVLLLVTPFLAAAWSLHP
Post Translational Modifications N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine. Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants. Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
Function Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
Protein Name Cd59 Glycoprotein
1f5 Antigen
20 Kda Homologous Restriction Factor
Hrf-20
Hrf20
Mac-Inhibitory Protein
Mac-Ip
Mem43 Antigen
Membrane Attack Complex Inhibition Factor
Macif
Membrane Inhibitor Of Reactive Lysis
Mirl
Protectin
Cd Antigen Cd59
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-5694530
Reactome: R-HSA-6798695
Reactome: R-HSA-6807878
Reactome: R-HSA-977606
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Secreted
Soluble Form Found In A Number Of Tissues
Alternative Antibody Names Anti-Cd59 Glycoprotein antibody
Anti-1f5 Antigen antibody
Anti-20 Kda Homologous Restriction Factor antibody
Anti-Hrf-20 antibody
Anti-Hrf20 antibody
Anti-Mac-Inhibitory Protein antibody
Anti-Mac-Ip antibody
Anti-Mem43 Antigen antibody
Anti-Membrane Attack Complex Inhibition Factor antibody
Anti-Macif antibody
Anti-Membrane Inhibitor Of Reactive Lysis antibody
Anti-Mirl antibody
Anti-Protectin antibody
Anti-Cd Antigen Cd59 antibody
Anti-CD59 antibody
Anti-MIC11 antibody
Anti-MIN1 antibody
Anti-MIN2 antibody
Anti-MIN3 antibody
Anti-MSK21 antibody

Information sourced from Uniprot.org

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