Anti-CD54-ICAM-1 antibody [F4-31C2] (STJ16101113)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
ImmunogenA BALB/c mouse was immunized with human umbilical cord vein endothelial cells (HUVEC). Fusion partner: X63Ag8/653.
General Information
| Short Description | Mouse monoclonal anti-CD54-ICAM-1 for use in FC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/IHC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | F4-31C2 |
| Isotype | IgG2ak |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (2-4 µg/ml for 30 min at RT; no antigen retrieval method has been established to date for staining of formalin fixed paraffin embedded secti |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Gene Symbol | ICAM1 |
| Gene ID | 3383 |
| Uniprot ID | ICAM1_HUMAN |
| Immunogen | A BALB/c mouse was immunized with human umbilical cord vein endothelial cells (HUVEC). Fusion partner: X63Ag8/653. |
Additional Info
| Post Translational Modifications | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell. |
| Protein Name | Intercellular Adhesion Molecule 1Icam-1Major Group Rhinovirus ReceptorCd Antigen Cd54 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-216083Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-877300 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Intercellular Adhesion Molecule 1 antibodyAnti-Icam-1 antibodyAnti-Major Group Rhinovirus Receptor antibodyAnti-Cd Antigen Cd54 antibodyAnti-ICAM1 antibody |
Information sourced from Uniprot.org