Anti-CD44 antibody [156-3C11] (STJ16101870)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
ImmunogenA BALB/c mouse was immunized with stimulated human leucocytes. Fusion partner: NS-1.
General Information
| Short Description | Mouse monoclonal anti-CD44 for use in FC, FUNC, WB, IF and IHC in African Green Monkey, Baboon and Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/FUNC/WB/IF/IHC |
| Host | Mouse |
| Reactivity | African Green Monkey/Baboon/Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 156-3C11 |
| Isotype | IgG2ak |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Functional studies (0, 02-2, 0 µg/ml without azide). Immunoblotting (1-2 µg/ml). Immunofluorescence ( 1-2 µg/ml). Immunohistology (formalin-fixed: 1-2 µg/ml for 30 min at RT; requires boiling ti |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Gene Symbol | CD44 |
| Gene ID | 960 |
| Uniprot ID | CD44_HUMAN |
| Immunogen | A BALB/c mouse was immunized with stimulated human leucocytes. Fusion partner: NS-1. |
Additional Info
| Post Translational Modifications | Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated.contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated.activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. |
| Function | Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion. |
| Protein Name | Cd44 AntigenCdw44EpicanExtracellular Matrix Receptor IiiEcmr-IiiGp90 Lymphocyte Homing/Adhesion ReceptorHutch-IHeparan Sulfate ProteoglycanHermes AntigenHyaluronate ReceptorPhagocytic Glycoprotein 1Pgp-1Phagocytic Glycoprotein IPgp-ICd Antigen Cd44 |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-2160916Reactome: R-HSA-6798695Reactome: R-HSA-877300 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionMicrovillusSecretedColocalizes With Actin In Membrane Protrusions At Wounding EdgesCo-Localizes With RdxEzr And Msn In MicrovilliLocalizes To Cholesterol-Rich Membrane-Bound Lipid Raft Domains |
| Alternative Antibody Names | Anti-Cd44 Antigen antibodyAnti-Cdw44 antibodyAnti-Epican antibodyAnti-Extracellular Matrix Receptor Iii antibodyAnti-Ecmr-Iii antibodyAnti-Gp90 Lymphocyte Homing/Adhesion Receptor antibodyAnti-Hutch-I antibodyAnti-Heparan Sulfate Proteoglycan antibodyAnti-Hermes Antigen antibodyAnti-Hyaluronate Receptor antibodyAnti-Phagocytic Glycoprotein 1 antibodyAnti-Pgp-1 antibodyAnti-Phagocytic Glycoprotein I antibodyAnti-Pgp-I antibodyAnti-Cd Antigen Cd44 antibodyAnti-CD44 antibodyAnti-LHR antibodyAnti-MDU2 antibodyAnti-MDU3 antibodyAnti-MIC4 antibody |
Information sourced from Uniprot.org