![Rat splenocytes were stained with Anti-Rat CD4 (domain 1 Conjugated) Monoclonal Antibody (PE Conjugated)[Used at 0.02 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/145464/292123/STJA0000611_1__72903.1631205954.jpg?c=1 "Rat splenocytes were stained with Anti-Rat CD4 (domain 1 Conjugated) Monoclonal Antibody (PE Conjugated)[Used at 0.02 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.")
![Rat splenocytes were stained with Anti-Rat CD4 (domain 1 Conjugated) Monoclonal Antibody (PE Conjugated)[Used at 0.02 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/145464/292123/STJA0000611_1__72903.1631205954.jpg?c=1 "Rat splenocytes were stained with Anti-Rat CD4 (domain 1 Conjugated) Monoclonal Antibody (PE Conjugated)[Used at 0.02 ยตg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.")
Anti-Cd4 antibody [OX-38] {PE} (STJA0000611)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationPE
IsotypeIgG2ak
General Information
| Short Description | Mouse monoclonal anti-T-cell surface glycoprotein CD4 for use in FC in Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC |
| Host | Mouse |
| Reactivity | Rat |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | OX-38 |
| Isotype | IgG2ak |
| Conjugation | PE |
| Concentration | 0.2 mg/mL |
| Formulation | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
| Storage Instruction | Store between 2-8ยฐC and protecting from prolonged exposure to light. Do not freeze this product. |
Target Information
| Gene Symbol | Cd4 |
| Gene ID | 24932 |
| Uniprot ID | CD4_RAT |
Additional Info
| Post Translational Modifications | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation plays an important role for CD4 internalization. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. |
| Protein Name | T-Cell Surface Glycoprotein Cd4T-Cell Surface Antigen T4/Leu-3W3/25 AntigenCd Antigen Cd4 |
| Database Links | Reactome: R-RNO-1462054Reactome: -RNO-202424Reactome: -RNO-202427Reactome: -RNO-202430Reactome: -RNO-202433Reactome: -RNO-389948Reactome: -RNO-449836Reactome: -RNO-8856825Reactome: -RNO-8856828 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLocalizes To Lipid Rafts |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd4 antibodyAnti-T-Cell Surface Antigen T4/Leu-3 antibodyAnti-W3/25 Antigen antibodyAnti-Cd Antigen Cd4 antibodyAnti-Cd4 antibody |
Information sourced from Uniprot.org