Host: | Rat |
Applications: | FC |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Short Description: | Rat monoclonal antibody anti-T-cell surface glycoprotein CD4 is suitable for use in Flow Cytometry research applications. |
Clonality: | Monoclonal |
Clone ID: | GK1.5 |
Conjugation: | FITC |
Isotype: | IgG2bk |
Formulation: | PBS with 0.05% Proclin300, 1% BSA |
Concentration: | 0.5 mg/mL |
Storage Instruction: | Recommend storing between 2-8°C and protecting from prolonged exposure to light. Do not freeze this product. |
Tissue Specificity | Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells. |
Post Translational Modifications | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation plays an important role for CD4 internalization. |
Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. |
Protein Name | T-Cell Surface Glycoprotein Cd4 T-Cell Differentiation Antigen L3t4 T-Cell Surface Antigen T4/Leu-3 Cd Antigen Cd4 |
Database Links | Reactome: R-MMU-1462054 Reactome: -MMU-202424 Reactome: -MMU-202427 Reactome: -MMU-202430 Reactome: -MMU-202433 Reactome: -MMU-389948 Reactome: -MMU-449836 Reactome: -MMU-8856825 Reactome: -MMU-8856828 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd4 antibody Anti-T-Cell Differentiation Antigen L3t4 antibody Anti-T-Cell Surface Antigen T4/Leu-3 antibody Anti-Cd Antigen Cd4 antibody Anti-Cd4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance