• Flow cytometry: 1X10^6 Raji cells (negative control, left) and Jurkat cells (right) were intracellularly-stained with CD3G Rabbit monoclonal antibody (STJ11103551, 2. 5 Mu g/mL orange line) or ABflo® 488 Rabbit IgG isotype control ( 2. 5 Mu g/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).
  • Immunofluorescence analysis of Jurkat cells using CD3G Rabbit monoclonal antibody (STJ11103551) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CD3G Rabbit monoclonal antibody (STJ11103551) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-CD3G antibody (83-182) [S1MR] (STJ11103551)

SKU:
STJ11103551

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF/FC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD3G (83-182) is suitable for use in Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
FC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD3G
Gene ID: 917
Uniprot ID: CD3G_HUMAN
Immunogen Region: 83-182
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 83-182 of human CD3G (P09693).
Immunogen Sequence: GMYQCKGSQNKSKPLQVYYR MCQNCIELNAATISGFLFAE IVSIFVLAVGVYFIAGQDGV RQSRASDKQTLLPNDQLYQP LKDREDDQYSHLQGNQLRRN
Post Translational Modifications Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. Phosphorylated also by PKC.leading to the TCR complex down-regulation. Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.
Protein Name T-Cell Surface Glycoprotein Cd3 Gamma Chain
T-Cell Receptor T3 Gamma Chain
Cd Antigen Cd3g
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-2029481
Reactome: R-HSA-2029482
Reactome: R-HSA-2029485
Reactome: R-HSA-389948
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-9664323
Reactome: R-HSA-9664422
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd3 Gamma Chain antibody
Anti-T-Cell Receptor T3 Gamma Chain antibody
Anti-Cd Antigen Cd3g antibody
Anti-CD3G antibody
Anti-T3G antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance