Anti-CD3G antibody (131-181) {FITC} (STJ500467)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationFITC
IsotypeIgG
ImmunogenSynthetic peptide taken within amino acid region 131-181 on human T-cell surface glycoprotein CD3 gamma protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-CD3G (131-181) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | FITC |
| Concentration | 0.55-0.75 ยตg/ยตl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:150 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20ยฐC for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | CD3G |
| Gene ID | 917 |
| Uniprot ID | CD3G_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 131-181 on human T-cell surface glycoprotein CD3 gamma protein. |
| Immunogen Region | 131-181 |
Additional Info
| Post Translational Modifications | Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. Phosphorylated also by PKC.leading to the TCR complex down-regulation. Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G. |
| Protein Name | T-Cell Surface Glycoprotein Cd3 Gamma ChainT-Cell Receptor T3 Gamma ChainCd Antigen Cd3g |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-202424Reactome: R-HSA-202427Reactome: R-HSA-202430Reactome: R-HSA-202433Reactome: R-HSA-2029481Reactome: R-HSA-2029482Reactome: R-HSA-2029485Reactome: R-HSA-389948Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9664323Reactome: R-HSA-9664422 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd3 Gamma Chain antibodyAnti-T-Cell Receptor T3 Gamma Chain antibodyAnti-Cd Antigen Cd3g antibodyAnti-CD3G antibodyAnti-T3G antibody |
Information sourced from Uniprot.org