• Immunofluorescence analysis of RAW264. 7 cells using CD3E Antigen antibody (STJ114289) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using CD3E Antigen antibody (STJ114289) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse thymus using CD3E Antigen antibody (STJ114289) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD3E Antigen antibody (STJ114289) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CD3E Antigen antibody (STJ114289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD3E antibody (111-207) (STJ114289)

SKU:
STJ114289

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD3E antigen (111-207) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD3E
Gene ID: 916
Uniprot ID: CD3E_HUMAN
Immunogen Region: 111-207
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 111-207 of human CD3E (NP_000724.1).
Immunogen Sequence: YLYLRARVCENCMEMDVMSV ATIVIVDICITGGLLLLVYY WSKNRKAKAKPVTRGAGAGG RQRGQNKERPPPVPNPDYEP IRKGQRDLYSGLNQRRI
Post Translational Modifications Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.
Protein Name T-Cell Surface Glycoprotein Cd3 Epsilon Chain
T-Cell Surface Antigen T3/Leu-4 Epsilon Chain
Cd Antigen Cd3e
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-389948
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd3 Epsilon Chain antibody
Anti-T-Cell Surface Antigen T3/Leu-4 Epsilon Chain antibody
Anti-Cd Antigen Cd3e antibody
Anti-CD3E antibody
Anti-T3E antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance