• Immunofluorescence analysis of THP-1 cells using CD38 Rabbit polyclonal antibody (STJ115571) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen cells using CD38 Rabbit polyclonal antibody (STJ115571) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CD38 in paraffin-embedded human spleen tissue using CD38 Rabbit polyclonal antibody (STJ115571) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0. 01M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using CD38 Rabbit polyclonal antibody (STJ115571) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Enhanced Kit. Negative control (NC) : Hep G2. Exposuretime: 30s.

Anti-CD38 antibody (43-300) (STJ115571)

SKU:
STJ115571

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD38 (43-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:2000-1:4000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD38
Gene ID: 952
Uniprot ID: CD38_HUMAN
Immunogen Region: 43-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 43-300 of human CD38 (NP_001766.2).
Immunogen Sequence: VPRWRQQWSGPGTTKRFPET VLARCVKYTEIHPEMRHVDC QSVWDAFKGAFISKHPCNIT EEDYQPLMKLGTQTVPCNKI LLWSRIKDLAHQFTQVQRDM FTLEDTLLGYLADDLTWCGE FNTSKINYQSCPDWRKDCSN NPVSVFWKTVSRRFAEAACD VVHVMLNGSRSKIFDKNSTF GSVEVHNLQPEKVQTLEAWV IHGGREDSRDLCQDPTIKEL ESIISKRNIQFSCKNIYRP
Tissue Specificity Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
Function Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion. Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+). Has cADPR hydrolase activity.
Protein Name Adp-Ribosyl Cyclase/Cyclic Adp-Ribose Hydrolase 1
2'-Phospho-Adp-Ribosyl Cyclase
2'-Phospho-Adp-Ribosyl Cyclase/2'-Phospho-Cyclic-Adp-Ribose Transferase
2'-Phospho-Cyclic-Adp-Ribose Transferase
Adp-Ribosyl Cyclase 1
Adprc 1
Cyclic Adp-Ribose Hydrolase 1
Cadpr Hydrolase 1
T10
Cd Antigen Cd38
Database Links Reactome: R-HSA-196807
Cellular Localisation Cell Surface
Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Adp-Ribosyl Cyclase/Cyclic Adp-Ribose Hydrolase 1 antibody
Anti-2'-Phospho-Adp-Ribosyl Cyclase antibody
Anti-2'-Phospho-Adp-Ribosyl Cyclase/2'-Phospho-Cyclic-Adp-Ribose Transferase antibody
Anti-2'-Phospho-Cyclic-Adp-Ribose Transferase antibody
Anti-Adp-Ribosyl Cyclase 1 antibody
Anti-Adprc 1 antibody
Anti-Cyclic Adp-Ribose Hydrolase 1 antibody
Anti-Cadpr Hydrolase 1 antibody
Anti-T10 antibody
Anti-Cd Antigen Cd38 antibody
Anti-CD38 antibody

Information sourced from Uniprot.org

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