Anti-CD36 antibody [EBS-CD-023] (STJ16101137)

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STJ16101137

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Host: Mouse
Applications: FC/IF/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-CD36 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: EBS-CD-023
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: PBS with 0.02% Sodium Azide.
Purification: Affinity purified from tissue culture.
Concentration: Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range: Flow cytometry (1-2 µg/million cells in 0, 1 ml for 30 min, at 4°C). Immunofluorescence (1-2 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; no antigen retrieval procedure is necessary for staining of formalin-fixed tissues).
Storage Instruction: Store for up to 1 year at 2-8°C upon receipt.
Gene Symbol: CD36
Gene ID: 948
Uniprot ID: CD36_HUMAN
Immunogen: A BALB/c mouse was immunized with gp88 purified from human platelets. Fusion partner: SP2/0.
Post Translational Modifications N-glycosylated and O-glycosylated with a ratio of 2:1. Palmitoylated by ZDHHC5. Palmitoylation is required for proper localization at the plasma membrane. Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome. Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids.
Function Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption. Mechanistically, binding of fatty acids activates downstream kinase LYN, which phosphorylates the palmitoyltransferase ZDHHC5 and inactivates it, resulting in the subsequent depalmitoylation of CD36 and caveolar endocytosis. In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway. Involved in oral fat perception and preferences. Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions. In taste receptor cells, mediates the induction of an increase in intracellular calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract. Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis. Receptor for thrombospondins, THBS1 and THBS2, mediating their antiangiogenic effects. Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting together with THBS1. As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome. Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
Protein Name Platelet Glycoprotein 4
Fatty Acid Translocase
Fat
Glycoprotein Iiib
Gpiiib
Leukocyte Differentiation Antigen Cd36
Pas Iv
Pas-4
Platelet Collagen Receptor
Platelet Glycoprotein Iv
Gpiv
Thrombospondin Receptor
Cd Antigen Cd36
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1236973
Reactome: R-HSA-1236974
Reactome: R-HSA-166058
Reactome: R-HSA-168188
Reactome: R-HSA-1989781
Reactome: R-HSA-3000471
Reactome: R-HSA-381340
Reactome: R-HSA-434313
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5686938
Reactome: R-HSA-6785807
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Membrane Raft
Golgi Apparatus
Apical Cell Membrane
Upon Ligand-Binding
Internalized Through Dynamin-Dependent Endocytosis
Alternative Antibody Names Anti-Platelet Glycoprotein 4 antibody
Anti-Fatty Acid Translocase antibody
Anti-Fat antibody
Anti-Glycoprotein Iiib antibody
Anti-Gpiiib antibody
Anti-Leukocyte Differentiation Antigen Cd36 antibody
Anti-Pas Iv antibody
Anti-Pas-4 antibody
Anti-Platelet Collagen Receptor antibody
Anti-Platelet Glycoprotein Iv antibody
Anti-Gpiv antibody
Anti-Thrombospondin Receptor antibody
Anti-Cd Antigen Cd36 antibody
Anti-CD36 antibody
Anti-GP3B antibody
Anti-GP4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance