Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4ยฐC overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-CD34 antibody (208-257 aa) (STJ92122)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CD34 at the amino acid range 208-257
STJ92122
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General Information

Short DescriptionRabbit polyclonal anti-Hematopoietic progenitor cell antigen CD34 (208-257 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
IHC-P 1:100-500
IF ICC 1:100-500
ELISA 1:5000-20000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCD34
Gene ID947
Uniprot IDCD34_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CD34 at the amino acid range 208-257
Immunogen Region208-257 aa
SpecificityCD34 Polyclonal Antibody detects endogenous levels of CD34 protein.

Additional Info

Post Translational Modifications Highly glycosylated. Phosphorylated on serine residues by PKC.
Function Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Protein Name Hematopoietic Progenitor Cell Antigen Cd34
Cd Antigen Cd34
Database Links Reactome: R-HSA-198933 P28906-1
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Hematopoietic Progenitor Cell Antigen Cd34 antibody
Anti-Cd Antigen Cd34 antibody
Anti-CD34 antibody

Information sourced from Uniprot.org

Citations

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