| Host: |
Mouse |
| Applications: |
FC/IF/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-CD33/SIGLE3 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
EBS-CD-022 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1k |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
Flow cytometry (1-2 µg/million cells in 0, 1 ml for 30 min, at 4°C). Immunofluorescence (1-2 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; no antigen retrieval procedure is known to date for formalin-fixed tissues). |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
CD33 |
| Gene ID: |
945 |
| Uniprot ID: |
CD33_HUMAN |
| Immunogen: |
A BALB/c mouse was immunized with stimulated human AML cells. Fusion partner: NS-1. |
| Tissue Specificity | Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells. |
| Post Translational Modifications | Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent. |
| Function | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K. |
| Protein Name | Myeloid Cell Surface Antigen Cd33Sialic Acid-Binding Ig-Like Lectin 3Siglec-3Gp67Cd Antigen Cd33 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Cd33m: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Cd33m: PeroxisomeCd33m Isoform Does Not Localize To Cell Surfaces But Instead Accumulates In Peroxisomes |
| Alternative Antibody Names | Anti-Myeloid Cell Surface Antigen Cd33 antibodyAnti-Sialic Acid-Binding Ig-Like Lectin 3 antibodyAnti-Siglec-3 antibodyAnti-Gp67 antibodyAnti-Cd Antigen Cd33 antibodyAnti-CD33 antibodyAnti-SIGLEC3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
