| Host: | Mouse |
| Applications: | IHC-P |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal CD33 (C2 Domain) antibody for use in IHC-P in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | PWS44 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b |
| Formulation: | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Purification: | Affinity purified |
| Dilution Range: | 1:100-200 |
| Storage Instruction: | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
| Gene Symbol: | CD33 |
| Gene ID: | 945 |
| Uniprot ID: | CD33_HUMAN |
| Immunogen Region: | C2 Domain |
| Specificity: | Positive Control: Spleen |
| Immunogen: | Prokaryotic recombinant protein corresponding to a region of the C2 domain of the human CD33 molecule |
| Tissue Specificity | Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells. |
| Post Translational Modifications | Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent. |
| Function | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K. |
| Protein Name | Myeloid Cell Surface Antigen Cd33Sialic Acid-Binding Ig-Like Lectin 3Siglec-3Gp67Cd Antigen Cd33 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Cd33m: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Cd33m: PeroxisomeCd33m Isoform Does Not Localize To Cell Surfaces But Instead Accumulates In Peroxisomes |
| Alternative Antibody Names | Anti-Myeloid Cell Surface Antigen Cd33 antibodyAnti-Sialic Acid-Binding Ig-Like Lectin 3 antibodyAnti-Siglec-3 antibodyAnti-Gp67 antibodyAnti-Cd Antigen Cd33 antibodyAnti-CD33 antibodyAnti-SIGLEC3 antibody |
Information sourced from Uniprot.org
![Anti-CD33 antibody [PWS44] (STJ16100448)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/149165/346790/STJ16100448_1__59494.1681998892.jpg?c=1 "Anti-CD33 antibody [PWS44] (STJ16100448)")
![Anti-CD33 antibody [PWS44] (STJ16101707)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/152213/347065/STJ16101707_1__11251.1681999325.jpg?c=1 "Anti-CD33 antibody [PWS44] (STJ16101707)")
