• Immunofluorescence analysis of TF-1 cells using SIGLEC3/CD33 Rabbit polyclonal antibody (STJ22989) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of RAW264. 7 cells using SIGLEC3/CD33 Rabbit polyclonal antibody (STJ22989) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of K-562 cells using SIGLEC3/CD33 Rabbit polyclonal antibody (STJ22989) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SIGLEC3/CD33 in paraffin-embedded mouse spleen using SIGLEC3/CD33 Rabbit polyclonal antibody (STJ22989) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SIGLEC3/CD33 Rabbit polyclonal antibody (STJ22989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CD33 antibody (49-259) (STJ22989)

SKU:
STJ22989

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SIGLEC3/CD33 (49-259) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD33
Gene ID: 945
Uniprot ID: CD33_HUMAN
Immunogen Region: 49-259
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 49-259 of human SIGLEC3/CD33 (NP_001763.3).
Immunogen Sequence: YYDKNSPVHGYWFREGAIIS RDSPVATNKLDQEVQEETQG RFRLLGDPSRNNCSLSIVDA RRRDNGSYFFRMERGSTKYS YKSPQLSVHVTDLTHRPKIL IPGTLEPGHSKNLTCSVSWA CEQGTPPIFSWLSAAPTSLG PRTTHSSVLIITPRPQDHGT NLTCQVKFAGAGVTTERTIQ LNVTYVPQNPTTGIFPGDGS GKQETRAGVVH
Tissue Specificity Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells.
Post Translational Modifications Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent.
Function Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
Protein Name Myeloid Cell Surface Antigen Cd33
Sialic Acid-Binding Ig-Like Lectin 3
Siglec-3
Gp67
Cd Antigen Cd33
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-6798695
Cellular Localisation Isoform Cd33m: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform Cd33m: Peroxisome
Cd33m Isoform Does Not Localize To Cell Surfaces But Instead Accumulates In Peroxisomes
Alternative Antibody Names Anti-Myeloid Cell Surface Antigen Cd33 antibody
Anti-Sialic Acid-Binding Ig-Like Lectin 3 antibody
Anti-Siglec-3 antibody
Anti-Gp67 antibody
Anti-Cd Antigen Cd33 antibody
Anti-CD33 antibody
Anti-SIGLEC3 antibody

Information sourced from Uniprot.org

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