Anti-CD279/PDCD1/PD1 antibody [J43V2] (STJA0027410)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
General Information
| Short Description | Mouse monoclonal Programmed Cell Death Protein 1 antibody for use in FC in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC |
| Host | Mouse |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | J43V2 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/ml |
| Purification | Protein A/G purified from cell culture supernatant. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | Pdcd1 |
| Gene ID | 18566 |
| Uniprot ID | PDCD1_MOUSE |
Additional Info
| Post Translational Modifications | Ubiquitinated at Lys-235 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains. Deubiquitinated and thus stabilized by USP5. Tyrosine phosphorylated at Tyr-225 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. Phosphorylation at Thr-236 promotes the recruitment of the deubiquitinase USP5. |
| Function | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival. |
| Protein Name | Programmed Cell Death Protein 1Protein Pd-1Mpd-1Cd Antigen Cd279 |
| Database Links | Reactome: R-MMU-389948 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Programmed Cell Death Protein 1 antibodyAnti-Protein Pd-1 antibodyAnti-Mpd-1 antibodyAnti-Cd Antigen Cd279 antibodyAnti-Pdcd1 antibodyAnti-Pd1 antibody |
Information sourced from Uniprot.org