Anti-CD279/PDCD1/PD1 antibody [29F.1A12] {FITC} (STJA0028608)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationFITC
IsotypeIgG2ak
General Information
| Short Description | Rat monoclonal Programmed Cell Death Protein 1 antibody for use in FC, IF and WB in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/WB |
| Host | Rat |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 29F.1A12 |
| Isotype | IgG2ak |
| Conjugation | FITC |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Formulation | PBS pH 7.4, 50% glycerol, 0.05% proclin300 |
| Storage Instruction | Store at 4°C for 12 months. Protect from light. Do not freeze. |
Target Information
| Gene Symbol | Pdcd1 |
| Gene ID | 18566 |
| Uniprot ID | PDCD1_MOUSE |
Additional Info
| Post Translational Modifications | Ubiquitinated at Lys-235 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains. Deubiquitinated and thus stabilized by USP5. Tyrosine phosphorylated at Tyr-225 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. Phosphorylation at Thr-236 promotes the recruitment of the deubiquitinase USP5. |
| Function | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival. |
| Protein Name | Programmed Cell Death Protein 1Protein Pd-1Mpd-1Cd Antigen Cd279 |
| Database Links | Reactome: R-MMU-389948 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Programmed Cell Death Protein 1 antibodyAnti-Protein Pd-1 antibodyAnti-Mpd-1 antibodyAnti-Cd Antigen Cd279 antibodyAnti-Pdcd1 antibodyAnti-Pd1 antibody |
Information sourced from Uniprot.org