![C57BL/6 murine splenocytes were stained with Anti-Mouse CD279 Monoclonal Antibody (PerCP/Cy5.5 Conjugated)[Used at 0.2 ยตg/10<sup>6</sup> cells dilution] and Anti-Mouse CD3 Monoclonal Antibody (FITC Conjugated).](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/145685/292343/STJA0000832_1__87976.1631206197.jpg?c=1 "C57BL/6 murine splenocytes were stained with Anti-Mouse CD279 Monoclonal Antibody (PerCP/Cy5.5 Conjugated)[Used at 0.2 ยตg/10<sup>6</sup> cells dilution] and Anti-Mouse CD3 Monoclonal Antibody (FITC Conjugated).")
![C57BL/6 murine splenocytes were stained with Anti-Mouse CD279 Monoclonal Antibody (PerCP/Cy5.5 Conjugated)[Used at 0.2 ยตg/10<sup>6</sup> cells dilution] and Anti-Mouse CD3 Monoclonal Antibody (FITC Conjugated).](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/145685/292343/STJA0000832_1__87976.1631206197.jpg?c=1 "C57BL/6 murine splenocytes were stained with Anti-Mouse CD279 Monoclonal Antibody (PerCP/Cy5.5 Conjugated)[Used at 0.2 ยตg/10<sup>6</sup> cells dilution] and Anti-Mouse CD3 Monoclonal Antibody (FITC Conjugated).")
Anti-CD279/Pdcd1 antibody [29F.1A12] {PerCP/Cyanine5.5} (STJA0000832)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationPerCP/Cyanine5.5
IsotypeIgG2ak
General Information
| Short Description | Rat monoclonal anti-Programmed cell death protein 1 for use in FC in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC |
| Host | Rat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 29F.1A12 |
| Isotype | IgG2ak |
| Conjugation | PerCP/Cyanine5.5 |
| Concentration | 0.2 mg/mL |
| Formulation | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
| Storage Instruction | Store between 2-8ยฐC and protecting from prolonged exposure to light. Do not freeze this product. |
Target Information
| Gene Symbol | Pdcd1 |
| Gene ID | 18566 |
| Uniprot ID | PDCD1_MOUSE |
Additional Info
| Tissue Specificity | Thymus-specific. |
| Post Translational Modifications | Ubiquitinated at Lys-235 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains. Deubiquitinated and thus stabilized by USP5. Tyrosine phosphorylated at Tyr-225 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. Phosphorylation at Thr-236 promotes the recruitment of the deubiquitinase USP5. |
| Function | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival. |
| Protein Name | Programmed Cell Death Protein 1Protein Pd-1Mpd-1Cd Antigen Cd279 |
| Database Links | Reactome: R-MMU-389948 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Programmed Cell Death Protein 1 antibodyAnti-Protein Pd-1 antibodyAnti-Mpd-1 antibodyAnti-Cd Antigen Cd279 antibodyAnti-Pdcd1 antibodyAnti-Pd1 antibody |
Information sourced from Uniprot.org